(1) If you provide an invalid emailaddress, you allow us to post your results publicly here. You also forego all intellectual property claims on your data. So please do provide a valid email everytime you use this software.
To test the tool on an example dataset, you can download sirna.csv and enter as a houdesholdgene GAPDH. The exact calculation is explained in the article Calculating Up-/Down- Regulations using qPCR/RTPCR. In case, any questions would remain, we would be happy to answer them: firstname.lastname@example.org.