Scientific

MK5 Microarray Data


Affected Genes - Ontology Breakdown - Gene Enrichment - Proteome Analysis

These are the micro-array results of a differential gene expression microarray experiments and the subsequent analysis steps performed on them. The up/down- regulation ratio was obtained by measuring WT cells against MK5 activated cells. See material and methods for technical information as well as the data usage policy.

Proteome Analysis

Below the proteome analysis is presented. The analysis methods are presented in the paper Propagating Differential Gene Expression in Protein Interaction Networks. If you are interested in the most reliable results choose the

  • value_type as 'ranked' since this provides a very robust analysis method.
  • intercation map as 'high confidence'
  • network_comparison type as 'Divided' since this leads to a natural grouping of similar proteins
  • filtered as 'true' since this will only use the microarray measurements that have a sufficiently large confidence level.

  • Navigation/Query Panel:
    Click on the attribute name to hide/unhide it. The green arrows can be used to shift columns left/right. Exact word match is written as =..., regular expressions can be matched with ~.... To select all values larger or equal than use >.... This would be <... for values smaller or equal than. To select all values within a specific range use [...,...].
    Rank Gene description Value Type Network Comparison Type Interaction Map Filtered red green network_comparison
    Results: HTML CSV LaTeX Showing element 2530 to 2579 of 25824 in total
    Interaction Map	High confidence
    Filtered 1
    Rank description Value Type Network Comparison Type red green network_comparison 317 dj756n5.1.1 (continues in em:al133324 as dj1161h23.3) (fragment). [source:sptrembl;acc:q9h430] Rooted Divided 95.0526 85.3206 1.11406 317 glutamate decarboxylase, 67 kda isoform (ec 4.1.1.15) (gad-67) (67 kda glutamic acid decarboxylase). [source:swissprot;acc:q99259] Rooted Subtracted 59.6399 51.9242 7.7157 317 melanoma-associated antigen 1 (mage-1 antigen) (antigen mz2-e). [source:swissprot;acc:p43355] Ranked Subtracted 88.2482 110.265 22.0168 317 nucleoside diphosphate kinase 3 (ec 2.7.4.6) (ndk 3) (ndp kinase 3) (nm23-h3) (dr-nm23). [source:swissprot;acc:q13232] Squared Divided 33982.3 43159.1 1.27005 317 splicing factor 3b subunit 3 (spliceosome associated protein 130) (sap 130) (sf3b130) (pre-mrna splicing factor sf3b 130 kda subunit). [source:swissprot;acc:q15393] Ranked Divided 224.445 204.044 1.09998 317 vav-3 protein. [source:swissprot;acc:q9ukw4] Measured Subtracted 6647.43 7706.18 1058.75 317 zinc finger homeobox protein 1b (smad interacting protein 1) (smadip1) (hrihfb2411). [source:swissprot;acc:o60315] Squared Subtracted 44848.2 52108.3 7260.1 318 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (ec 2.3.1.51) (1- agp acyltransferase 3) (1-agpat 3) (lysophosphatidic acid acyltransferase-gamma) (lpaat-gamma) (1-acylglycerol-3-phosphate o- acyltransferase 3). [source:swissprot;acc:q9nrz7] Measured Subtracted 7485.08 8543.66 1058.58 318 chromatin accessibility complex protein 1 (chrac-1) (chrac-15) (huchrac15) (dna polymerase epsilon subunit p15). [source:swissprot;acc:q9nrg0] Rooted Subtracted 38.8223 46.4975 7.6752 318 melanoma-associated antigen 10 (mage-10 antigen). [source:swissprot;acc:p43363] Ranked Subtracted 88.2482 110.265 22.0168 318 mitochondrial 60s ribosomal protein l3 (l3mt). [source:swissprot;acc:p09001] Ranked Divided 242.011 266.18 1.09987 318 mitochondrial import receptor subunit tom20 homolog (mitochondrial 20 kda outer membrane protein) (outer mitochondrial membrane receptor tom20). [source:swissprot;acc:q15388] Squared Divided 46739.9 59134.5 1.26518 318 myosin heavy chain, skeletal muscle, extraocular (myhc-eo). [source:swissprot;acc:q9ukx3] Rooted Divided 95.0526 85.3206 1.11406 318 nuclear dna-binding protein; small unique nuclear receptor corepressor; c1d dna-binding protein. [source:refseq;acc:nm_006333] Measured Divided 34190.9 40795.8 1.19318 318 transcription factor 8 (nil-2-a zinc finger protein) (negative regulator of il2). [source:swissprot;acc:p37275] Squared Subtracted 44848.2 52108.3 7260.1 319 1-acyl-sn-glycerol-3-phosphate acyltransferase delta (ec 2.3.1.51) (1- agp acyltransferase 4) (1-agpat 4) (lysophosphatidic acid acyltransferase-delta) (lpaat-delta) (1-acylglycerol-3-phosphate o- acyltransferase 4). [source:swissprot;acc:q9nrz5] Measured Subtracted 7477 8532.02 1055.02 319 60s ribosomal protein l19. [source:swissprot;acc:p14118] Ranked Divided 240.73 264.729 1.09969 319 b9 protein; likely ortholog of mouse endothelial precursor protein b9. [source:refseq;acc:nm_015681] Rooted Subtracted 53.4993 45.9227 7.5766 319 maguk p55 subfamily member 3 (mpp3 protein) (discs, large homolog 3). [source:swissprot;acc:q13368] Squared Subtracted 37147.5 44372.1 7224.6 319 melanoma-associated antigen b1 (mage-b1 antigen) (mage-xp antigen) (dss-ahc critical interval mage superfamily 10) (dam10). [source:swissprot;acc:p43366] Ranked Subtracted 88.2482 110.265 22.0168 319 peroxisomal farnesylated protein (33 kda housekeeping protein) (peroxin 19). [source:swissprot;acc:p40855] Squared Divided 46739.9 59134.5 1.26518 319 ras-related gtp binding a; ras-related gtp-binding protein. [source:refseq;acc:nm_006570] Measured Divided 20911 24908.5 1.19117 319 saccharomyces cerevisiae nip7p homolog. [source:refseq;acc:nm_016101] Rooted Divided 49.0663 54.4978 1.1107 320 40s ribosomal protein s29. [source:swissprot;acc:p30054] Rooted Divided 50.2784 45.294 1.11005 320 carboxypeptidase b precursor. [source:refseq;acc:nm_020361] Rooted Subtracted 77.5801 70.0182 7.5619 320 coatomer alpha subunit (alpha-coat protein) (alpha-cop) (hepcop) (hep-cop) [contains: xenin (xenopsin-related peptide); proxenin]. [source:swissprot;acc:p53621] Squared Subtracted 49280.5 42272.7 7007.8 320 epithelial-cadherin precursor (e-cadherin) (uvomorulin) (cadherin-1) (cam 120/80). [source:swissprot;acc:p12830] Ranked Divided 241.892 265.96 1.0995 320 epoxide hydrolase 1 (ec 3.3.2.3) (microsomal epoxide hydrolase) (epoxide hydratase). [source:swissprot;acc:p07099] Squared Divided 87971.9 69563.1 1.26463 320 melanoma-associated antigen d2 (mage-d2 antigen) (breast cancer associated gene 1 protein) (bcg-1) (11b6) (hepatocellular carcinoma associated protein jcl-1). [source:swissprot;acc:q9unf1] Ranked Subtracted 88.2482 110.265 22.0168 320 ras-related gtp binding c; rag c protein. [source:refseq;acc:nm_022157] Measured Divided 20911 24908.5 1.19117 320 steroid hormone receptor err2 (estrogen-related receptor, beta) (err-beta) (estrogen receptor-like 2) (err beta-2). [source:swissprot;acc:o95718] Measured Subtracted 10263.8 11318.6 1054.8 321 c367g8.3 (novel protein similar to rpl23a (60s ribosomal protein l23a)) (60s ribosomal protein l23a like). [source:sptrembl;acc:q9br02] Ranked Divided 241.892 265.959 1.09949 321 fibulin-1 precursor. [source:swissprot;acc:p23142] Squared Subtracted 44407.1 51387.4 6980.3 321 mast cell carboxypeptidase a precursor (ec 3.4.17.1) (mc-cpa) (carboxypeptidase a3). [source:swissprot;acc:p15088] Rooted Subtracted 77.58 70.0181 7.5619 321 putative nucleoside diphosphate kinase (ec 2.7.4.6) (ndk) (ndp kinase). [source:swissprot;acc:o60361] Squared Divided 33526 42390.2 1.2644 321 ras-related gtp binding b short isoform; gtp-binding protein ragb. [source:refseq;acc:nm_006064] Measured Divided 20911 24908.5 1.19117 321 signal transducing adaptor molecule 1; signal transducing adaptor molecule. [source:refseq;acc:nm_003473] Measured Subtracted 11797.1 10750.2 1046.9 321 trophinin. [source:swissprot;acc:q12816] Ranked Subtracted 88.2482 110.265 22.0168 321 u6 snrna-associated sm-like protein lsm7. [source:swissprot;acc:q9uk45] Rooted Divided 55.9231 62.0565 1.10968 322 anillin, actin binding protein (scraps homolog, drosophila); anillin (drosophila scraps homolog), actin binding protein. [source:refseq;acc:nm_018685] Measured Divided 20911 24908.5 1.19117 322 carboxypeptidase b precursor. [source:refseq;acc:nm_020361] Squared Subtracted 25326.4 18354.1 6972.3 322 deoxyribonuclease ii beta isoform 1 precursor; deoxyribonuclease ii beta; endonuclease dlad. [source:refseq;acc:nm_021233] Ranked Divided 86.7483 78.8995 1.09948 322 nucleoside diphosphate kinase a (ec 2.7.4.6) (ndk a) (ndp kinase a) (tumor metastatic process-associated protein) (metastasis inhibition factor nm23) (nm23-h1). [source:swissprot;acc:p15531] Squared Divided 33522.8 42384.8 1.26436 322 plasma carboxypeptidase b2 isoform a preproprotein; carboxypeptidase u; thrombin-activatable fibrinolysis inhibitor; carboxypeptidase b-like protein; thrombin-activable fibrinolysis inhibitor. [source:refseq;acc:nm_001872] Rooted Subtracted 77.5797 70.0179 7.5618 322 pyruvate carboxylase, mitochondrial precursor (ec 6.4.1.1) (pyruvic carboxylase) (pcb). [source:swissprot;acc:p11498] Measured Subtracted 9304.83 10347.3 1042.47 322 rgs19-interacting protein 1 (gaip c-terminus interacting protein gipc) (rgs-gaip interacting protein) (tax interaction protein 2) (tip-2). [source:swissprot;acc:o14908] Rooted Divided 60.594 67.2244 1.10942 322 ribonucleoside-diphosphate reductase m2 chain (ec 1.17.4.1) (ribonucleotide reductase small chain). [source:swissprot;acc:p31350] Ranked Subtracted 231.969 210.064 21.905 323 40s ribosomal protein s14 (pro2640). [source:swissprot;acc:p06366] Ranked Subtracted 242.238 264.11 21.872 323 60s ribosomal protein l23a. [source:swissprot;acc:p29316] Ranked Divided 241.884 265.945 1.09947 323 cholinephosphate cytidylyltransferase a (ec 2.7.7.15) (phosphorylcholine transferase a) (ctp:phosphocholine cytidylyltransferase a) (ct a) (cct a) (cct-alpha). [source:swissprot;acc:p49585] Rooted Divided 50.3803 45.4193 1.10923 Legend: - Rank is the rank after comparing the two networks - Value Type describes how the microarray measurement was processed. Ranked indicates that we sorted all measurements and use the rank as red and green value. Measured is the normal measurement. Squared and Rooted are the square and square root respecitcely. This might be valueable to compensate for non linear light distributions. - Network Comparison Type describes whether we divided the red and green types or whether we sutractcted them - Interaction Map specifies whether we used only the hig hconfidence protein-protein interactions or also included the low confidence interactions. - Filtered specifies whether we only included the high confidence microarry measurements or not.

    - http://analysis.yellowcouch.org/