Scientific

MK5 Microarray Data


Affected Genes - Ontology Breakdown - Gene Enrichment - Proteome Analysis

These are the micro-array results of a differential gene expression microarray experiments and the subsequent analysis steps performed on them. The up/down- regulation ratio was obtained by measuring WT cells against MK5 activated cells. See material and methods for technical information as well as the data usage policy.

Proteome Analysis

Below the proteome analysis is presented. The analysis methods are presented in the paper Propagating Differential Gene Expression in Protein Interaction Networks. If you are interested in the most reliable results choose the

  • value_type as 'ranked' since this provides a very robust analysis method.
  • intercation map as 'high confidence'
  • network_comparison type as 'Divided' since this leads to a natural grouping of similar proteins
  • filtered as 'true' since this will only use the microarray measurements that have a sufficiently large confidence level.

  • Navigation/Query Panel:
    Click on the attribute name to hide/unhide it. The green arrows can be used to shift columns left/right. Exact word match is written as =..., regular expressions can be matched with ~.... To select all values larger or equal than use >.... This would be <... for values smaller or equal than. To select all values within a specific range use [...,...].
    Rank Gene description Value Type Network Comparison Type Interaction Map Filtered red green network_comparison
    Results: HTML CSV LaTeX Showing element 2675 to 2724 of 25824 in total
    Interaction Map	High confidence
    Filtered 1
    Rank description Value Type Network Comparison Type red green network_comparison 335 chromodomain helicase dna binding protein 5. [source:refseq;acc:nm_015557] Ranked Divided 312 284 1.09859 335 histone acetyltransferase type b subunit 2 (retinoblastoma binding protein p46) (retinoblastoma-binding protein 7) (rbbp-7). [source:swissprot;acc:q16576] Rooted Subtracted 52.7146 60.0088 7.2942 335 multisynthetase complex auxiliary component p43 [contains: endothelial-monocyte activating polypeptide ii (emap-ii) (small inducible cytokine subfamily e member 1)]. [source:swissprot;acc:q12904] Ranked Subtracted 223.273 202.423 20.85 335 probable atp-dependent 61 kda nucleolar rna helicase (dead-box protein 21). [source:swissprot;acc:q9ny93] Measured Divided 3494.31 4129.6 1.18181 335 ribosomal protein l24-like; 60s ribosomal protein l30 isolog; my024 protein; homolog of yeast ribosomal like protein 24. [source:refseq;acc:nm_016304] Squared Divided 30651.3 38253.3 1.24802 335 t-lak cell-originated protein kinase; spermatogenesis-related protein kinase; pdz-binding kinase; pbk; mapkk-like protein kinase; serine/threonine protein kinase. [source:refseq;acc:nm_018492] Measured Subtracted 1631.67 2664.55 1032.88 336 60s ribosomal protein l11. [source:swissprot;acc:p39026] Rooted Divided 50.3167 45.4063 1.10814 336 acid ceramidase precursor (ec 3.5.1.23) (acylsphingosine deacylase) (n-acylsphingosine amidohydrolase) (ac) (putative 32 kda heart protein) (php32). [source:swissprot;acc:q13510] Squared Subtracted 36109.8 42811.6 6701.8 336 cyclin-dependent kinase 2-associated protein 1 (cdk2-associated protein 1) (putative oral cancer suppressor) (deleted in oral cancer- 1) (doc-1). [source:swissprot;acc:o14519] Ranked Divided 312 284 1.09859 336 dna polymerase epsilon p12 subunit (dna polymerase epsilon subunit 4). [source:swissprot;acc:q9nr33] Measured Divided 3751.3 4431.34 1.18128 336 j kappa-recombination signal binding protein (rbp-j kappa). [source:swissprot;acc:q06330] Squared Divided 46785.7 58383.2 1.24789 336 kh domain containing, rna binding, signal transduction associated 3; sam68-like phosphotyrosine protein, t-star. [source:refseq;acc:nm_006558] Measured Subtracted 1631.67 2664.55 1032.88 336 polymerase (dna directed) iota; rad30 (s. cerevisiae) homolog b; polymerase (dna-directed), iota. [source:refseq;acc:nm_007195] Rooted Subtracted 59.4073 66.7002 7.2929 336 rna polymerase i associated factor 53. [source:refseq;acc:nm_022490] Ranked Subtracted 241.045 261.874 20.829 337 60s ribosomal protein l12. [source:swissprot;acc:p30050] Ranked Divided 243.583 267.527 1.0983 337 carboxypeptidase b precursor. [source:refseq;acc:nm_020361] Rooted Divided 77.5801 70.0182 1.108 337 homeobox protein hox-c4 (hox-3e) (cp19). [source:swissprot;acc:p09017] Measured Divided 20446.1 24136.4 1.18049 337 human immunodeficiency virus type i enhancer binding protein 3. [source:refseq;acc:nm_024503] Squared Divided 46785.7 58383.2 1.24789 337 kh domain containing, rna binding, signal transduction associated 1; gap-associated tyrosine phosphoprotein p62 (sam68). [source:refseq;acc:nm_006559] Measured Subtracted 1631.67 2664.55 1032.88 337 nuclear receptor co-repressor/hdac3 complex subunit tblr1 (tbl1- related protein 1). [source:swissprot;acc:q9bzk7] Squared Subtracted 28895.6 35572.8 6677.2 337 paxillin. [source:swissprot;acc:p49023] Rooted Subtracted 59.4073 66.7002 7.2929 337 source of immunodominant mhc-associated peptides; homolog of yeast stt3 gene. [source:refseq;acc:nm_178862] Ranked Subtracted 200.691 179.88 20.811 338 60s ribosomal protein l12 like protein. [source:sptrembl;acc:o60886] Ranked Divided 243.583 267.528 1.0983 338 homeobox protein hox-b4 (hox-2f) (hox-2.6). [source:swissprot;acc:p17483] Measured Divided 20446.1 24136.4 1.18049 338 importin beta-1 subunit (karyopherin beta-1 subunit) (nuclear factor p97) (importin 90). [source:swissprot;acc:q14974] Squared Subtracted 28895.6 35572.8 6677.2 338 kh domain-containing, rna-binding, signal transduction-associated protein 2; sam68-like mammalian protein 1. [source:refseq;acc:nm_152688] Measured Subtracted 1631.67 2664.55 1032.88 338 mast cell carboxypeptidase a precursor (ec 3.4.17.1) (mc-cpa) (carboxypeptidase a3). [source:swissprot;acc:p15088] Rooted Divided 77.58 70.0181 1.108 338 metastasis-associated protein mta1. [source:swissprot;acc:q13330] Rooted Subtracted 52.7557 60.035 7.2793 338 oligosaccharyl transferase stt3 subunit homolog (b5) (integral membrane protein 1) (tmc). [source:swissprot;acc:p46977] Ranked Subtracted 200.691 179.88 20.811 338 zinc finger protein 40 (human immunodeficiency virus type i enhancer- binding protein 1) (hiv-ep1) (major histocompatibility complex binding protein 1) (mbp-1) (positive regulatory domain ii binding factor 1) (prdii-bf1). [source:swissprot;acc:p15822] Squared Divided 46785.7 58383.2 1.24789 339 chromatin assembly factor 1 subunit c (caf-1 subunit c) (chromatin assembly factor i p48 subunit) (caf-i 48 kda subunit) (caf-ip48) (retinoblastoma binding protein p48) (retinoblastoma-binding protein 4) (rbbp-4) (msi1 protein homolog). [source:swissprot;acc:q09028] Rooted Subtracted 52.7936 60.0592 7.2656 339 dj999l4.1 (novel protein similar to ribosomal protein l12 (rpl12)). [source:sptrembl;acc:q9nq02] Ranked Divided 243.583 267.527 1.0983 339 dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kda subunit precursor (ec 2.4.1.119) (oligosaccharyl transferase 48 kda subunit) (ddost 48 kda subunit). [source:swissprot;acc:p39656] Ranked Subtracted 200.691 179.88 20.811 339 homeobox protein hox-c5 (hox-3d) (cp11). [source:swissprot;acc:q00444] Measured Divided 20446.1 24136.4 1.18049 339 human immunodeficiency virus type i enhancer-binding protein 2 (hiv-ep2). [source:swissprot;acc:p31629] Squared Divided 46785.7 58383.2 1.24789 339 neuralized-like. [source:refseq;acc:nm_004210] Measured Subtracted 1657.68 2690.25 1032.57 339 plasma carboxypeptidase b2 isoform a preproprotein; carboxypeptidase u; thrombin-activatable fibrinolysis inhibitor; carboxypeptidase b-like protein; thrombin-activable fibrinolysis inhibitor. [source:refseq;acc:nm_001872] Rooted Divided 77.5797 70.0179 1.108 339 transducin beta-like 1x protein (transducin-beta-like 1, x-linked). [source:swissprot;acc:o60907] Squared Subtracted 28895.6 35572.8 6677.2 340 28s ribosomal protein s9, mitochondrial precursor (mrp-s9). [source:swissprot;acc:p82933] Ranked Subtracted 239.75 260.544 20.794 340 homeobox protein hox-c6 (hox-3c) (hho.c8) (cp25). [source:swissprot;acc:p09630] Measured Divided 20446.1 24136.4 1.18049 340 myelin gene expression factor 2. [source:refseq;acc:nm_016132] Ranked Divided 235.383 258.476 1.09811 340 nuclear matrix protein nmp200 related to splicing factor prp19. [source:refseq;acc:nm_014502] Rooted Divided 58.4926 64.8047 1.10791 340 ribonucleotide reductase m2 b (tp53 inducible); p53-inducible ribonucleotide reductase small subunit 2 homolog. [source:refseq;acc:nm_015713] Squared Divided 32117 40054 1.24713 340 signal transducing adaptor molecule 2; stam-like protein containing sh3 and itam domains 2. [source:refseq;acc:nm_005843] Measured Subtracted 11743.3 10712.3 1031 340 transcription factor dp-2 (e2f dimerization partner 2). [source:swissprot;acc:q14188] Rooted Subtracted 45.7414 52.932 7.1906 340 transducin beta-like 1y protein (transducin-beta-like 1, y-linked). [source:swissprot;acc:q9bq87] Squared Subtracted 28895.6 35572.8 6677.2 341 60s ribosomal protein l9. [source:swissprot;acc:p32969] Ranked Divided 240.243 263.798 1.09805 341 acetyl-coa carboxylase 2 (ec 6.4.1.2) (acc-beta) [includes: biotin carboxylase (ec 6.3.4.14)]. [source:swissprot;acc:o00763] Squared Subtracted 31646.1 38288.7 6642.6 341 homeobox protein hox-d4 (hox-4b) (hox-5.1) (hho.c13). [source:swissprot;acc:p09016] Measured Divided 20446.1 24136.4 1.18049 341 lupus la protein (sjogren syndrome type b antigen) (ss-b) (la ribonucleoprotein) (la autoantigen). [source:swissprot;acc:p05455] Rooted Divided 51.9812 57.5858 1.10782 Legend: - Rank is the rank after comparing the two networks - Value Type describes how the microarray measurement was processed. Ranked indicates that we sorted all measurements and use the rank as red and green value. Measured is the normal measurement. Squared and Rooted are the square and square root respecitcely. This might be valueable to compensate for non linear light distributions. - Network Comparison Type describes whether we divided the red and green types or whether we sutractcted them - Interaction Map specifies whether we used only the hig hconfidence protein-protein interactions or also included the low confidence interactions. - Filtered specifies whether we only included the high confidence microarry measurements or not.

    - http://analysis.yellowcouch.org/