Scientific

MK5 Microarray Data


Affected Genes - Ontology Breakdown - Gene Enrichment - Proteome Analysis

These are the micro-array results of a differential gene expression microarray experiments and the subsequent analysis steps performed on them. The up/down- regulation ratio was obtained by measuring WT cells against MK5 activated cells. See material and methods for technical information as well as the data usage policy.

Proteome Analysis

Below the proteome analysis is presented. The analysis methods are presented in the paper Propagating Differential Gene Expression in Protein Interaction Networks. If you are interested in the most reliable results choose the

  • value_type as 'ranked' since this provides a very robust analysis method.
  • intercation map as 'high confidence'
  • network_comparison type as 'Divided' since this leads to a natural grouping of similar proteins
  • filtered as 'true' since this will only use the microarray measurements that have a sufficiently large confidence level.

  • Navigation/Query Panel:
    Click on the attribute name to hide/unhide it. The green arrows can be used to shift columns left/right. Exact word match is written as =..., regular expressions can be matched with ~.... To select all values larger or equal than use >.... This would be <... for values smaller or equal than. To select all values within a specific range use [...,...].
    Rank Gene description Value Type Network Comparison Type Interaction Map Filtered red green network_comparison
    Results: HTML CSV LaTeX Showing element 2675 to 2724 of 12912 in total
    Network Comparison Type	Divided
    Interaction Map High confidence
    Filtered 1
    Rank description Value Type red green network_comparison 669 synapsin i (brain protein 4.1). [source:swissprot;acc:p17600] Ranked 229.072 216.576 1.0577 669 u6 snrna-associated sm-like protein lsm4 (glycine-rich protein) (grp). [source:swissprot;acc:q9y4z0] Squared 18548.7 21798.2 1.17519 670 autoantigen ngp-1. [source:swissprot;acc:q13823] Ranked 250.302 236.817 1.05694 670 dna-directed rna polymerase i 40 kda polypeptide (ec 2.7.7.6) (rpa40) (rpa39). [source:swissprot;acc:o15160] Rooted 51.2122 47.5637 1.07671 670 dok-like protein. [source:refseq;acc:nm_024872] Squared 28911 33971.3 1.17503 670 tubby related protein 3 (tubby-like protein 3). [source:swissprot;acc:o75386] Measured 6374.65 7145.59 1.12094 671 docking protein 2 (p56(dok-2)) (downstream of tyrosine kinase 2). [source:swissprot;acc:o60496] Squared 28911 33971.3 1.17503 671 nucleotide-binding protein 1 (nbp 1). [source:swissprot;acc:p53384] Ranked 210.739 199.398 1.05688 671 proline synthetase co-transcribed bacterial homolog protein. [source:swissprot;acc:o94903] Rooted 68.6822 73.8955 1.0759 671 vacuolar protein sorting 35 (vesicle protein sorting 35) (hvps35) (maternal-embryonic 3). [source:swissprot;acc:q96qk1] Measured 6374.65 7145.59 1.12094 672 dna-directed rna polymerase i 135 kda polypeptide (ec 2.7.7.6) (rna polymerase i subunit 2) (rpa135). [source:swissprot;acc:q9h9y6] Rooted 51.4203 47.7936 1.07588 672 docking protein 1 (p62(dok)) (downstream of tyrosine kinase 1) (pp62). [source:swissprot;acc:q99704] Squared 28911 33971.3 1.17503 672 nucleotide binding protein 2 (nbp 2). [source:swissprot;acc:q9y5y2] Ranked 210.739 199.398 1.05688 672 protein kinase c, zeta type (ec 2.7.1.37) (npkc-zeta). [source:swissprot;acc:q05513] Measured 6374.65 7145.59 1.12094 673 peripheral plasma membrane protein cask (ec 2.7.1.-) (hcask) (calcium/calmodulin-dependent serine protein kinase) (lin-2 homolog). [source:swissprot;acc:o14936] Ranked 226.497 214.355 1.05664 673 poly(a) polymerase alpha (ec 2.7.7.19) (pap) (polynucleotide adenylyltransferase alpha) (fragment). [source:swissprot;acc:p51003] Rooted 132.156 122.835 1.07588 673 transcription factor btf3 (rna polymerase b transcription factor 3). [source:swissprot;acc:p20290] Measured 6933.17 7766.54 1.1202 673 tyrosine-protein kinase frk (ec 2.7.1.112) (nuclear tyrosine protein kinase rak). [source:swissprot;acc:p42685] Squared 28911 33971.3 1.17503 674 apoptosis inhibitor fksg2. [source:swissprot;acc:q9hau6] Squared 42467.5 36156.7 1.17454 674 exocyst complex component sec5. [source:swissprot;acc:q96kp1] Rooted 132.114 122.802 1.07583 674 hypoxia-inducible factor-3 alpha isoform a; inhibitory pas domain protein. [source:refseq;acc:nm_152794] Ranked 226.497 214.355 1.05664 674 sprouty homolog 4 (spry-4). [source:swissprot;acc:q9c004] Measured 5549.2 6215.42 1.12006 675 poly(a) polymerase gamma (ec 2.7.7.19) (pap gamma) (polynucleotide adenylyltransferase gamma) (srp rna 3' adenylating enzyme). [source:swissprot;acc:q9bwt3] Rooted 132.063 122.761 1.07577 675 single-minded homolog 1. [source:swissprot;acc:p81133] Ranked 226.497 214.355 1.05664 675 sprouty homolog 3 (spry-3). [source:swissprot;acc:o43610] Measured 5549.2 6215.42 1.12006 675 [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial precursor (ec 2.7.1.115) (branched-chain alpha-ketoacid dehydrogenase kinase) (bckdhkin) (bckd-kinase). [source:swissprot;acc:o14874] Squared 22347.6 26247.3 1.1745 676 cleavage and polyadenylation specificity factor, 73 kda subunit (cpsf 73 kda subunit). [source:swissprot;acc:q9ukf6] Rooted 132.467 123.158 1.07559 676 dipeptidylpeptidase 10; dipeptidyl peptidase iv-related protein 3. [source:refseq;acc:nm_020868] Squared 22337.3 26234.1 1.17445 676 dna-directed rna polymerase ii 19 kda polypeptide (ec 2.7.7.6) (rpb7). [source:swissprot;acc:p52433] Ranked 232.469 245.613 1.05654 676 sprouty homolog 1 (spry-1) (fragment). [source:swissprot;acc:o43609] Measured 5549.2 6215.42 1.12006 677 cytochrome c oxidase assembly protein cox11, mitochondrial precursor. [source:swissprot;acc:q9y6n1] Squared 22335.8 26232.2 1.17445 677 lymphoid enhancer binding factor 1 (lef-1) (t cell-specific transcription factor 1-alpha) (tcf1-alpha). [source:swissprot;acc:q9uju2] Rooted 59.2629 63.7418 1.07558 677 sprouty homolog 2 (spry-2). [source:swissprot;acc:o43597] Measured 5549.2 6215.42 1.12006 677 u6 snrna-associated sm-like protein lsm4 (glycine-rich protein) (grp). [source:swissprot;acc:q9y4z0] Ranked 228.944 216.745 1.05628 678 myelin gene expression factor 2. [source:refseq;acc:nm_016132] Rooted 56.8369 52.8585 1.07527 678 sorting nexin 5. [source:swissprot;acc:q9y5x3] Ranked 208.482 220.175 1.05609 678 surfeit locus protein 1. [source:swissprot;acc:q15526] Squared 22335.8 26232.2 1.17445 678 transcription factor btf3 homolog 3. [source:swissprot;acc:q13892] Measured 6938.22 7770.65 1.11998 679 adenylosuccinate synthase-like 1. [source:refseq;acc:nm_152328] Measured 6503.1 7282.94 1.11992 679 dipeptidyl aminopeptidase-like protein 6 (dipeptidylpeptidase vi) (dipeptidylpeptidase 6) (dipeptidyl peptidase iv like protein) (dipeptidyl aminopeptidase-related protein) (dppx). [source:swissprot;acc:p42658] Squared 22337.3 26234.1 1.17445 679 homeobox protein hox-c4 (hox-3e) (cp19). [source:swissprot;acc:p09017] Ranked 132.413 125.389 1.05602 679 putative pre-mrna splicing factor rna helicase (atp-dependent rna helicase #3) (deah-box protein 16). [source:swissprot;acc:o60231] Rooted 61.5558 66.1861 1.07522 680 adenylosuccinate synthetase (ec 6.3.4.4) (imp--aspartate ligase) (adss) (ampsase). [source:swissprot;acc:p30520] Measured 6503.03 7282.5 1.11986 680 homeobox protein hox-b4 (hox-2f) (hox-2.6). [source:swissprot;acc:p17483] Ranked 132.413 125.389 1.05602 680 seprase (ec 3.4.21.-) (fibroblast activation protein alpha) (integral membrane serine protease) (170-kda melanoma membrane-bound gelatinase). [source:swissprot;acc:q12884] Squared 22336.8 26233.5 1.17445 680 t54 protein. [source:swissprot;acc:q92917] Rooted 61.5558 66.1861 1.07522 681 cox15 homolog isoform 2 precursor; cytochrome c oxidase subunit 15; cytochrome c oxidase assembly protein. [source:refseq;acc:nm_004376] Squared 22335.8 26232.2 1.17445 681 homeobox protein hox-c5 (hox-3d) (cp11). [source:swissprot;acc:q00444] Ranked 132.413 125.389 1.05602 681 oligopeptide transporter, kidney isoform (peptide transporter 2) (kidney h+/peptide cotransporter) (solute carrier family 15, member 2). [source:swissprot;acc:q16348] Rooted 80.2562 86.277 1.07502 681 ribosomal protein l24-like; 60s ribosomal protein l30 isolog; my024 protein; homolog of yeast ribosomal like protein 24. [source:refseq;acc:nm_016304] Measured 7170.83 8029.64 1.11976 Legend: - Rank is the rank after comparing the two networks - Value Type describes how the microarray measurement was processed. Ranked indicates that we sorted all measurements and use the rank as red and green value. Measured is the normal measurement. Squared and Rooted are the square and square root respecitcely. This might be valueable to compensate for non linear light distributions. - Network Comparison Type describes whether we divided the red and green types or whether we sutractcted them - Interaction Map specifies whether we used only the hig hconfidence protein-protein interactions or also included the low confidence interactions. - Filtered specifies whether we only included the high confidence microarry measurements or not.

    - http://analysis.yellowcouch.org/