Scientific

MK5 Microarray Data


Affected Genes - Ontology Breakdown - Gene Enrichment - Proteome Analysis

These are the micro-array results of a differential gene expression microarray experiments and the subsequent analysis steps performed on them. The up/down- regulation ratio was obtained by measuring WT cells against MK5 activated cells. See material and methods for technical information as well as the data usage policy.

Proteome Analysis

Below the proteome analysis is presented. The analysis methods are presented in the paper Propagating Differential Gene Expression in Protein Interaction Networks. If you are interested in the most reliable results choose the

  • value_type as 'ranked' since this provides a very robust analysis method.
  • intercation map as 'high confidence'
  • network_comparison type as 'Divided' since this leads to a natural grouping of similar proteins
  • filtered as 'true' since this will only use the microarray measurements that have a sufficiently large confidence level.

  • Navigation/Query Panel:
    Click on the attribute name to hide/unhide it. The green arrows can be used to shift columns left/right. Exact word match is written as =..., regular expressions can be matched with ~.... To select all values larger or equal than use >.... This would be <... for values smaller or equal than. To select all values within a specific range use [...,...].
    Rank Gene description Value Type Network Comparison Type Filtered Interaction Map red green network_comparison
    Results: HTML CSV LaTeX Showing element 1551 to 1600 of 12912 in total
    Network Comparison Type	Divided
    Filtered 1
    Interaction Map High confidence
    Rank description Value Type red green network_comparison 388 serine racemase (ec 5.1.1.-). [source:swissprot;acc:q9gzt4] Rooted 50.3345 45.6718 1.10209 388 small nuclear ribonucleoprotein e (snrnp-e) (sm protein e) (sm-e) (sme). [source:swissprot;acc:p08578] Ranked 227.575 208.756 1.09015 389 60s ribosomal protein l34. [source:swissprot;acc:p49207] Rooted 50.3345 45.6718 1.10209 389 arginyl-trna synthetase-like; arginine-trna ligase. [source:refseq;acc:nm_020320] Squared 31819.7 39222.2 1.23264 389 probable atp-dependent rna helicase p54 (oncogene rck) (dead-box protein 6). [source:swissprot;acc:p26196] Measured 4533.74 5279.4 1.16447 389 small nuclear ribonucleoprotein f (snrnp-f) (sm protein f) (sm-f) (smf). [source:swissprot;acc:q15356] Ranked 227.575 208.756 1.09015 390 60s ribosomal protein l13, mitochondrial (l13mt). [source:swissprot;acc:q9byd1] Ranked 241.828 263.62 1.09011 390 nuclear receptor co-repressor/hdac3 complex subunit tblr1 (tbl1- related protein 1). [source:swissprot;acc:q9bzk7] Squared 28895.6 35572.8 1.23108 390 protein transport protein sec61 alpha subunit isoform 2 (sec61 alpha- 2). [source:swissprot;acc:q9y2r3] Rooted 50.3345 45.6718 1.10209 390 rw1 protein (fragment). [source:swissprot;acc:q92545] Measured 4533.74 5279.4 1.16447 391 40s ribosomal protein s16. [source:swissprot;acc:p17008] Ranked 241.828 263.62 1.09011 391 importin beta-1 subunit (karyopherin beta-1 subunit) (nuclear factor p97) (importin 90). [source:swissprot;acc:q14974] Squared 28895.6 35572.8 1.23108 391 protein transport protein sec61 alpha subunit isoform 1 (sec61 alpha- 1). [source:swissprot;acc:p38378] Rooted 50.3345 45.6718 1.10209 391 tar dna-binding protein-43 (tdp-43). [source:swissprot;acc:q13148] Measured 3658.76 3144.39 1.16358 392 60s ribosomal protein l13a (23 kda highly basic protein). [source:swissprot;acc:p40429] Ranked 241.83 263.615 1.09008 392 60s ribosomal protein l3-like. [source:swissprot;acc:q92901] Rooted 50.3313 45.6913 1.10155 392 atp-dependent rna helicase mgc2835; atp-dependent rna helicase; apoptosis related protein apr-5; dead box helicase 97 kda. [source:refseq;acc:nm_024072] Measured 3641.89 4236.95 1.16339 392 transducin beta-like 1x protein (transducin-beta-like 1, x-linked). [source:swissprot;acc:o60907] Squared 28895.6 35572.8 1.23108 393 dna-directed rna polymerase ii largest subunit (ec 2.7.7.6) (rpb1). [source:swissprot;acc:p24928] Rooted 49.8842 45.2875 1.1015 393 eukaryotic translation initiation factor 4e (eif-4e) (eif4e) (mrna cap-binding protein) (eif-4f 25 kda subunit). [source:swissprot;acc:p06730] Measured 4490.28 5222.25 1.16301 393 spliceosomal u5 snrnp-specific 15 kda protein (dim1 protein homolog) (thioredoxin-like u5 snrnp protein u5-15kd). [source:swissprot;acc:o14834] Ranked 225.719 207.072 1.09005 393 transducin beta-like 1y protein (transducin-beta-like 1, y-linked). [source:swissprot;acc:q9bq87] Squared 28895.6 35572.8 1.23108 394 bai1-associated protein 3; bai-associated protein 3. [source:refseq;acc:nm_003933] Measured 4490.28 5222.25 1.16301 394 dipeptidylpeptidase 9; dipeptidyl peptidase 9; dipeptidyl peptidase iv-related protein-2. [source:refseq;acc:nm_139159] Ranked 218.827 238.407 1.08948 394 mad protein (max dimerizer). [source:swissprot;acc:q05195] Squared 17248.4 21214 1.22991 394 pre-mrna splicing factor prp17 (hprp17) (eh-binding protein 3) (ehb3). [source:swissprot;acc:o60508] Rooted 58.6533 64.5958 1.10132 395 dipeptidyl peptidase 8 isoform 2; dipeptidyl peptidase 8. [source:refseq;acc:nm_017743] Ranked 218.827 238.407 1.08948 395 max interacting protein 1 (mxi1 protein). [source:swissprot;acc:p50539] Squared 17254.4 21218.1 1.22972 395 protein translation factor sui1 homolog (sui1iso1). [source:swissprot;acc:p41567] Rooted 50.0423 45.4523 1.10098 395 splicing factor 4 isoform b; rna-binding protein. [source:refseq;acc:nm_021164] Measured 4532.73 5269.33 1.16251 396 a-raf proto-oncogene serine/threonine-protein kinase (ec 2.7.1.-) (a-raf-1) (proto-oncogene pks). [source:swissprot;acc:p10398] Rooted 116.797 106.111 1.10071 396 max-interacting transcriptional repressor mad4 (max-associated protein 4) (max dimerization protein 4). [source:swissprot;acc:q14582] Squared 17256.5 21219.6 1.22966 396 putative eukaryotic translation initiation factor 1a (eif-1a) (eif- 4c). [source:swissprot;acc:o75642] Ranked 240.801 262.34 1.08945 396 ribonucleotide reductase m2 b (tp53 inducible); p53-inducible ribonucleotide reductase small subunit 2 homolog. [source:refseq;acc:nm_015713] Measured 6563.65 7627.29 1.16205 397 max dimerization protein 3; likely ortholog of mouse max dimerization protein 3. [source:refseq;acc:nm_031300] Squared 17257.5 21220.3 1.22963 397 nuclear protein skip (ski-interacting protein) (snw1 protein) (nuclear receptor coactivator ncoa-62). [source:swissprot;acc:q13573] Measured 5367.04 6233.12 1.16137 397 raf proto-oncogene serine/threonine-protein kinase (ec 2.7.1.-) (raf-1) (c-raf). [source:swissprot;acc:p04049] Rooted 116.792 106.107 1.1007 397 t-complex protein 1, gamma subunit (tcp-1-gamma) (cct-gamma). [source:swissprot;acc:p49368] Ranked 110.689 101.613 1.08932 398 imp2 inner mitochondrial membrane protease-like; inner mitochondrial membrane peptidase 2 like. [source:refseq;acc:nm_032549] Squared 18929.1 23222.8 1.22683 398 muscle-specific beta 1 integrin binding protein. [source:refseq;acc:nm_014446] Ranked 218.818 238.358 1.0893 398 serine/threonine protein phosphatase 2a, 56 kda regulatory subunit, epsilon isoform (pp2a, b subunit, b' epsilon isoform) (pp2a, b subunit, b56 epsilon isoform) (pp2a, b subunit, pr61 epsilon isoform) (pp2a, b subunit, r5 epsilon isoform). [source:swissprot;acc:q16537] Rooted 116.707 106.044 1.10055 398 xpa-binding protein 2 (hcnp protein) (pp3898). [source:swissprot;acc:q9hcs7] Measured 5084.26 5904.1 1.16125 399 clathrin heavy chain 1 (clh-17). [source:swissprot;acc:q00610] Ranked 207.889 226.226 1.08821 399 cyclin g2. [source:swissprot;acc:q16589] Rooted 116.707 106.044 1.10055 399 microsomal signal peptidase 18 kda subunit (ec 3.4.-.-) (spase 18 kda subunit) (spc18) (endopeptidase sp18). [source:swissprot;acc:p21378] Squared 18929.1 23222.8 1.22683 399 neuronal acetylcholine receptor protein, alpha-7 chain precursor. [source:swissprot;acc:p36544] Measured 5201.41 4479.76 1.16109 400 adenylate cyclase, type vi (ec 4.6.1.1) (atp pyrophosphate-lyase) (ca(2+)-inhibitable adenylyl cyclase). [source:swissprot;acc:o43306] Measured 5201.41 4479.76 1.16109 400 cyclin g1 (cyclin g). [source:swissprot;acc:p51959] Rooted 116.707 106.044 1.10055 400 glycogen [starch] synthase, muscle (ec 2.4.1.11). [source:swissprot;acc:p13807] Squared 39478.8 48419 1.22646 400 phosducin-like protein (phlp). [source:swissprot;acc:q13371] Ranked 207.908 226.218 1.08807 Legend: - Rank is the rank after comparing the two networks - Value Type describes how the microarray measurement was processed. Ranked indicates that we sorted all measurements and use the rank as red and green value. Measured is the normal measurement. Squared and Rooted are the square and square root respecitcely. This might be valueable to compensate for non linear light distributions. - Network Comparison Type describes whether we divided the red and green types or whether we sutractcted them - Interaction Map specifies whether we used only the hig hconfidence protein-protein interactions or also included the low confidence interactions. - Filtered specifies whether we only included the high confidence microarry measurements or not.

    - http://analysis.yellowcouch.org/