Scientific

MK5 Microarray Data


Affected Genes - Ontology Breakdown - Gene Enrichment - Proteome Analysis

These are the micro-array results of a differential gene expression microarray experiments and the subsequent analysis steps performed on them. The up/down- regulation ratio was obtained by measuring WT cells against MK5 activated cells. See material and methods for technical information as well as the data usage policy.

Proteome Analysis

Below the proteome analysis is presented. The analysis methods are presented in the paper Propagating Differential Gene Expression in Protein Interaction Networks. If you are interested in the most reliable results choose the

  • value_type as 'ranked' since this provides a very robust analysis method.
  • intercation map as 'high confidence'
  • network_comparison type as 'Divided' since this leads to a natural grouping of similar proteins
  • filtered as 'true' since this will only use the microarray measurements that have a sufficiently large confidence level.

  • Navigation/Query Panel:
    Click on the attribute name to hide/unhide it. The green arrows can be used to shift columns left/right. Exact word match is written as =..., regular expressions can be matched with ~.... To select all values larger or equal than use >.... This would be <... for values smaller or equal than. To select all values within a specific range use [...,...].
    Rank Gene description Value Type Network Comparison Type Interaction Map Filtered red green network_comparison
    Results: HTML CSV LaTeX Showing element 1575 to 1624 of 12912 in total
    Network Comparison Type	Divided
    Interaction Map High confidence
    Filtered 1
    Rank description Value Type red green network_comparison 394 mad protein (max dimerizer). [source:swissprot;acc:q05195] Squared 17248.4 21214 1.22991 394 pre-mrna splicing factor prp17 (hprp17) (eh-binding protein 3) (ehb3). [source:swissprot;acc:o60508] Rooted 58.6533 64.5958 1.10132 395 dipeptidyl peptidase 8 isoform 2; dipeptidyl peptidase 8. [source:refseq;acc:nm_017743] Ranked 218.827 238.407 1.08948 395 max interacting protein 1 (mxi1 protein). [source:swissprot;acc:p50539] Squared 17254.4 21218.1 1.22972 395 protein translation factor sui1 homolog (sui1iso1). [source:swissprot;acc:p41567] Rooted 50.0423 45.4523 1.10098 395 splicing factor 4 isoform b; rna-binding protein. [source:refseq;acc:nm_021164] Measured 4532.73 5269.33 1.16251 396 a-raf proto-oncogene serine/threonine-protein kinase (ec 2.7.1.-) (a-raf-1) (proto-oncogene pks). [source:swissprot;acc:p10398] Rooted 116.797 106.111 1.10071 396 max-interacting transcriptional repressor mad4 (max-associated protein 4) (max dimerization protein 4). [source:swissprot;acc:q14582] Squared 17256.5 21219.6 1.22966 396 putative eukaryotic translation initiation factor 1a (eif-1a) (eif- 4c). [source:swissprot;acc:o75642] Ranked 240.801 262.34 1.08945 396 ribonucleotide reductase m2 b (tp53 inducible); p53-inducible ribonucleotide reductase small subunit 2 homolog. [source:refseq;acc:nm_015713] Measured 6563.65 7627.29 1.16205 397 max dimerization protein 3; likely ortholog of mouse max dimerization protein 3. [source:refseq;acc:nm_031300] Squared 17257.5 21220.3 1.22963 397 nuclear protein skip (ski-interacting protein) (snw1 protein) (nuclear receptor coactivator ncoa-62). [source:swissprot;acc:q13573] Measured 5367.04 6233.12 1.16137 397 raf proto-oncogene serine/threonine-protein kinase (ec 2.7.1.-) (raf-1) (c-raf). [source:swissprot;acc:p04049] Rooted 116.792 106.107 1.1007 397 t-complex protein 1, gamma subunit (tcp-1-gamma) (cct-gamma). [source:swissprot;acc:p49368] Ranked 110.689 101.613 1.08932 398 imp2 inner mitochondrial membrane protease-like; inner mitochondrial membrane peptidase 2 like. [source:refseq;acc:nm_032549] Squared 18929.1 23222.8 1.22683 398 muscle-specific beta 1 integrin binding protein. [source:refseq;acc:nm_014446] Ranked 218.818 238.358 1.0893 398 serine/threonine protein phosphatase 2a, 56 kda regulatory subunit, epsilon isoform (pp2a, b subunit, b' epsilon isoform) (pp2a, b subunit, b56 epsilon isoform) (pp2a, b subunit, pr61 epsilon isoform) (pp2a, b subunit, r5 epsilon isoform). [source:swissprot;acc:q16537] Rooted 116.707 106.044 1.10055 398 xpa-binding protein 2 (hcnp protein) (pp3898). [source:swissprot;acc:q9hcs7] Measured 5084.26 5904.1 1.16125 399 clathrin heavy chain 1 (clh-17). [source:swissprot;acc:q00610] Ranked 207.889 226.226 1.08821 399 cyclin g2. [source:swissprot;acc:q16589] Rooted 116.707 106.044 1.10055 399 microsomal signal peptidase 18 kda subunit (ec 3.4.-.-) (spase 18 kda subunit) (spc18) (endopeptidase sp18). [source:swissprot;acc:p21378] Squared 18929.1 23222.8 1.22683 399 neuronal acetylcholine receptor protein, alpha-7 chain precursor. [source:swissprot;acc:p36544] Measured 5201.41 4479.76 1.16109 400 adenylate cyclase, type vi (ec 4.6.1.1) (atp pyrophosphate-lyase) (ca(2+)-inhibitable adenylyl cyclase). [source:swissprot;acc:o43306] Measured 5201.41 4479.76 1.16109 400 cyclin g1 (cyclin g). [source:swissprot;acc:p51959] Rooted 116.707 106.044 1.10055 400 glycogen [starch] synthase, muscle (ec 2.4.1.11). [source:swissprot;acc:p13807] Squared 39478.8 48419 1.22646 400 phosducin-like protein (phlp). [source:swissprot;acc:q13371] Ranked 207.908 226.218 1.08807 401 adenylate cyclase, type v (ec 4.6.1.1) (atp pyrophosphate-lyase) (adenylyl cyclase) (fragment). [source:swissprot;acc:o95622] Measured 5201.41 4479.76 1.16109 401 axonemal dynein light intermediate polypeptide 1 (inner dynein arm light chain, axonemal) (hp28). [source:swissprot;acc:o14645] Squared 33044.3 40524.8 1.22638 401 replication protein a 70 kda dna-binding subunit (rp-a) (rf-a) (replication factor-a protein 1) (single-stranded dna-binding protein). [source:swissprot;acc:p27694] Ranked 207.908 226.218 1.08807 401 serine/threonine protein phosphatase 2a, 56 kda regulatory subunit, alpha isoform (pp2a, b subunit, b' alpha isoform) (pp2a, b subunit, b56 alpha isoform) (pp2a, b subunit, pr61 alpha isoform) (pp2a, b subunit, r5 alpha isoform). [source:swissprot;acc:q15172] Rooted 116.707 106.044 1.10055 402 endo-beta-n-acetylglucosaminidase. [source:refseq;acc:nm_022759] Squared 39472 48393.1 1.22601 402 eukaryotic translation initiation factor 6 (eif-6) (b4 integrin interactor) (cab) (p27(bbp)) (b(2)gcn homolog). [source:swissprot;acc:p56537] Rooted 50.1918 55.2294 1.10037 402 leucine-rich repeat-containing protein 15 precursor (hlib). [source:swissprot;acc:q8tf66] Measured 5201.41 4479.76 1.16109 402 replication protein a 32 kda subunit (rp-a) (rf-a) (replication factor-a protein 2). [source:swissprot;acc:p15927] Ranked 207.908 226.218 1.08807 403 fem-1 homolog b; fem-1-like death receptor binding protein; fem-1 (c.elegans) homolog b. [source:refseq;acc:nm_015322] Measured 5201.41 4479.76 1.16109 403 glycogen [starch] synthase, liver (ec 2.4.1.11). [source:swissprot;acc:p54840] Squared 39463.9 48362.1 1.22548 403 nucleotide-binding protein 1 (nbp 1). [source:swissprot;acc:p53384] Rooted 59.6834 65.6741 1.10037 403 phosducin (phd) (33 kda phototransducing protein) (meka protein). [source:swissprot;acc:p20941] Ranked 207.908 226.218 1.08807 404 adenylosuccinate synthetase (ec 6.3.4.4) (imp--aspartate ligase) (adss) (ampsase). [source:swissprot;acc:p30520] Squared 26819.1 32855.4 1.22507 404 dna repair protein rad52 homolog. [source:swissprot;acc:p43351] Ranked 207.908 226.218 1.08807 404 leucine-rich repeat protein lrrc3 precursor. [source:swissprot;acc:q9by71] Measured 5201.41 4479.76 1.16109 404 nucleotide binding protein 2 (nbp 2). [source:swissprot;acc:q9y5y2] Rooted 59.6834 65.6741 1.10037 405 adenylosuccinate synthase-like 1. [source:refseq;acc:nm_152328] Squared 26822.2 32858.1 1.22503 405 b-raf proto-oncogene serine/threonine-protein kinase (ec 2.7.1.-) (p94) (v-raf murine sarcoma viral oncogene homolog b1). [source:swissprot;acc:p15056] Rooted 116.576 105.948 1.10031 405 clathrin heavy chain 2 (clh-22). [source:swissprot;acc:p53675] Ranked 207.931 226.209 1.0879 405 laminin gamma-1 chain precursor (laminin b2 chain). [source:swissprot;acc:p11047] Measured 5201.41 4479.76 1.16109 406 60s ribosomal protein l12. [source:swissprot;acc:p30050] Rooted 49.9858 45.4433 1.09996 406 serine protease htra2, mitochondrial precursor (ec 3.4.21.-) (high temperature requirement protein a2) (htra2) (omi stress-regulated endoprotease) (serine proteinase omi). [source:swissprot;acc:o43464] Squared 18995.7 23245.6 1.22373 406 thymic stromal co-transporter. [source:refseq;acc:nm_033051] Measured 5201.41 4479.76 1.16109 406 transcription factor dp-1 (e2f dimerization partner 1) (drtf1- polypeptide-1) (drtf1). [source:swissprot;acc:q14186] Ranked 265.634 244.304 1.08731 Legend: - Rank is the rank after comparing the two networks - Value Type describes how the microarray measurement was processed. Ranked indicates that we sorted all measurements and use the rank as red and green value. Measured is the normal measurement. Squared and Rooted are the square and square root respecitcely. This might be valueable to compensate for non linear light distributions. - Network Comparison Type describes whether we divided the red and green types or whether we sutractcted them - Interaction Map specifies whether we used only the hig hconfidence protein-protein interactions or also included the low confidence interactions. - Filtered specifies whether we only included the high confidence microarry measurements or not.

    - http://analysis.yellowcouch.org/