Scientific

MK5 Microarray Data


Affected Genes - Ontology Breakdown - Gene Enrichment - Proteome Analysis

These are the micro-array results of a differential gene expression microarray experiments and the subsequent analysis steps performed on them. The up/down- regulation ratio was obtained by measuring WT cells against MK5 activated cells. See material and methods for technical information as well as the data usage policy.

Proteome Analysis

Below the proteome analysis is presented. The analysis methods are presented in the paper Propagating Differential Gene Expression in Protein Interaction Networks. If you are interested in the most reliable results choose the

  • value_type as 'ranked' since this provides a very robust analysis method.
  • intercation map as 'high confidence'
  • network_comparison type as 'Divided' since this leads to a natural grouping of similar proteins
  • filtered as 'true' since this will only use the microarray measurements that have a sufficiently large confidence level.

  • Navigation/Query Panel:
    Click on the attribute name to hide/unhide it. The green arrows can be used to shift columns left/right. Exact word match is written as =..., regular expressions can be matched with ~.... To select all values larger or equal than use >.... This would be <... for values smaller or equal than. To select all values within a specific range use [...,...].
    Rank Gene description Value Type Network Comparison Type Interaction Map Filtered red green network_comparison
    Results: HTML CSV LaTeX Showing element 1730 to 1779 of 12912 in total
    Network Comparison Type	Divided
    Interaction Map High confidence
    Filtered 1
    Rank description Value Type red green network_comparison 433 laminin gamma-1 chain precursor (laminin b2 chain). [source:swissprot;acc:p11047] Rooted 54.4927 49.6125 1.09837 433 mitochondrial processing peptidase alpha subunit, mitochondrial precursor (ec 3.4.24.64) (alpha-mpp) (p-55) (ha1523). [source:swissprot;acc:q10713] Measured 5244.88 6036.38 1.15091 433 programmed cell death protein 8, mitochondrial precursor (ec 1.-.-.-) (apoptosis-inducing factor). [source:swissprot;acc:o95831] Ranked 212.547 229.411 1.07934 434 splicing factor 3b subunit 1 (spliceosome associated protein 155) (sap 155) (sf3b155) (pre-mrna splicing factor sf3b 155 kda subunit). [source:swissprot;acc:o75533] Squared 18306.1 22230.6 1.21438 434 t-box transcription factor tbx6 (t-box protein 6). [source:swissprot;acc:o95947] Ranked 212.547 229.411 1.07934 434 thymic stromal co-transporter. [source:refseq;acc:nm_033051] Rooted 54.4927 49.6125 1.09837 434 ubiquinol-cytochrome c reductase complex core protein i, mitochondrial precursor (ec 1.10.2.2). [source:swissprot;acc:p31930] Measured 5246.38 6037.37 1.15077 435 60s ribosomal protein l19. [source:swissprot;acc:p14118] Rooted 50.832 46.3081 1.09769 435 cytochrome b5 reductase b5r.2. [source:refseq;acc:nm_016229] Squared 26598.9 32283.5 1.21372 435 polymerase (dna directed) iota; rad30 (s. cerevisiae) homolog b; polymerase (dna-directed), iota. [source:refseq;acc:nm_007195] Ranked 221.522 205.286 1.07909 435 proline synthetase co-transcribed bacterial homolog protein. [source:swissprot;acc:o94903] Measured 5233.86 6022.6 1.1507 436 arg/abl-interacting protein 2 isoform 2; arg binding protein 2. [source:refseq;acc:nm_021069] Measured 6230.94 7168.2 1.15042 436 cytochrome b5 reductase 1 (b5r.1). [source:refseq;acc:nm_016243] Squared 26598.9 32283.5 1.21372 436 paxillin. [source:swissprot;acc:p49023] Ranked 221.522 205.286 1.07909 436 putative glialblastoma cell differentiation-related; putative glialblastoma cell differentiation-related protein. [source:refseq;acc:nm_016172] Rooted 210.928 192.165 1.09764 437 cdc42-interacting protein 4 (thyroid receptor interacting protein 10) (trip-10). [source:swissprot;acc:q15642] Ranked 209.921 226.459 1.07878 437 nadh-cytochrome b5 reductase (ec 1.6.2.2) (b5r). [source:swissprot;acc:p00387] Squared 26598.9 32283.5 1.21372 437 suppressor of swi4 1 homolog (ssf-1) (peter pan homolog). [source:swissprot;acc:q9nq55] Measured 3779.92 4348.48 1.15042 437 zinc finger protein t86. [source:swissprot;acc:o00488] Rooted 51.0466 56.024 1.09751 438 arg/abl-interacting protein 2 isoform 2; arg binding protein 2. [source:refseq;acc:nm_021069] Squared 28863.2 35031.5 1.21371 438 bag-family molecular chaperone regulator-3 (bcl-2 binding athanogene- 3) (bag-3) (bcl-2-binding protein bis) (docking protein cair-1). [source:swissprot;acc:o95817] Rooted 55.1867 60.5522 1.09722 438 n-terminal acetyltransferase complex ard1 subunit homolog (ec 2.3.1.-). [source:swissprot;acc:p41227] Ranked 233.723 216.674 1.07869 438 swi/snf-related, matrix-associated, actin-dependent regulator of chromatin subfamily f member 1 (swi-snf complex protein p270) (b120). [source:swissprot;acc:o14497] Measured 6252.58 7192.88 1.15039 439 40s ribosomal protein s9. [source:swissprot;acc:p46781] Ranked 238.986 257.784 1.07866 439 cell division cycle protein 23; anaphase-promoting complex subunit 8. [source:refseq;acc:nm_004661] Squared 51616.7 42588.9 1.21198 439 dok-like protein. [source:refseq;acc:nm_024872] Rooted 63.2134 69.352 1.09711 439 programmed cell death protein 6 (probable calcium-binding protein alg- 2). [source:swissprot;acc:o75340] Measured 6252.58 7192.88 1.15039 440 calcium-dependent protease, small subunit (calpain regulatory subunit) (calcium-activated neutral proteinase) (canp). [source:swissprot;acc:p04632] Measured 6251.91 7192.08 1.15038 440 dna topoisomerase ii, alpha isozyme (ec 5.99.1.3). [source:swissprot;acc:p11388] Squared 26348.4 31925.8 1.21168 440 docking protein 2 (p56(dok-2)) (downstream of tyrosine kinase 2). [source:swissprot;acc:o60496] Rooted 63.2134 69.352 1.09711 440 highly expressed in cancer, rich in leucine heptad repeats. [source:refseq;acc:nm_006101] Ranked 231.802 249.983 1.07843 441 dna topoisomerase ii, beta isozyme (ec 5.99.1.3). [source:swissprot;acc:q02880] Squared 26348.7 31924.3 1.21161 441 docking protein 1 (p62(dok)) (downstream of tyrosine kinase 1) (pp62). [source:swissprot;acc:q99704] Rooted 63.2134 69.352 1.09711 441 grancalcin. [source:swissprot;acc:p28676] Measured 6251.96 7192.13 1.15038 441 grb2-related adaptor protein. [source:swissprot;acc:q13588] Ranked 223.457 207.222 1.07835 442 centromere protein j; centrosomal p4.1-associated protein; lyst-interacting protein lip1; lag-3-associated protein. [source:refseq;acc:nm_018451] Squared 51286.6 42334.5 1.21146 442 e2a-pbx1-associated protein; putative 47 kda protein. [source:refseq;acc:nm_020140] Ranked 223.802 207.55 1.0783 442 sorcin (22 kda protein) (cp-22) (v19). [source:swissprot;acc:p30626] Measured 6251.99 7192.17 1.15038 442 tyrosine-protein kinase frk (ec 2.7.1.112) (nuclear tyrosine protein kinase rak). [source:swissprot;acc:p42685] Rooted 63.2134 69.352 1.09711 443 60s ribosomal protein l10a (csa-19). [source:swissprot;acc:p53025] Rooted 50.1766 45.7369 1.09707 443 brg1-binding protein eld/osa1; eld (eyelid)/osa protein. [source:refseq;acc:nm_020732] Measured 6252.27 7192.51 1.15038 443 neuron navigator 2 isoform l; retinoic acid inducible in neuroblastoma; pore membrane and/or filament interacting like protein 2; helicase helad1. [source:refseq;acc:nm_145117] Ranked 223.79 207.539 1.0783 443 translation initiation factor if-2, mitochondrial precursor (if-2mt) (if-2(mt)). [source:swissprot;acc:p46199] Squared 26472.8 32067.7 1.21135 444 60s ribosomal protein l10 (qm protein) (tumor suppressor qm) (laminin receptor homolog). [source:swissprot;acc:p27635] Rooted 50.1636 45.7275 1.09701 444 neuron navigator 1; neuron navigator-1; pore membrane and/or filament interacting like protein 3. [source:refseq;acc:nm_020443] Ranked 223.801 207.549 1.0783 444 probable rrna processing protein ebp2 (ebna1 binding protein 2) (nucleolar protein p40). [source:swissprot;acc:q99848] Measured 3758.67 4322.87 1.15011 444 ubiquitin-conjugating enzyme e2 e1 (ec 6.3.2.19) (ubiquitin-protein ligase e1) (ubiquitin carrier protein e1) (ubch6). [source:swissprot;acc:p51965] Squared 58831 48584.2 1.21091 445 ad24 protein. [source:refseq;acc:nm_022451] Measured 3753.71 4315.95 1.14978 445 neuron navigator 3; pore membrane and/or filament interacting like protein 1; steerin 3. [source:refseq;acc:nm_014903] Ranked 223.801 207.549 1.0783 445 ribosomal protein l10-like protein. [source:refseq;acc:nm_080746] Rooted 50.1635 45.7274 1.09701 Legend: - Rank is the rank after comparing the two networks - Value Type describes how the microarray measurement was processed. Ranked indicates that we sorted all measurements and use the rank as red and green value. Measured is the normal measurement. Squared and Rooted are the square and square root respecitcely. This might be valueable to compensate for non linear light distributions. - Network Comparison Type describes whether we divided the red and green types or whether we sutractcted them - Interaction Map specifies whether we used only the hig hconfidence protein-protein interactions or also included the low confidence interactions. - Filtered specifies whether we only included the high confidence microarry measurements or not.

    - http://analysis.yellowcouch.org/