Scientific

MK5 Microarray Data


Affected Genes - Ontology Breakdown - Gene Enrichment - Proteome Analysis

These are the micro-array results of a differential gene expression microarray experiments and the subsequent analysis steps performed on them. The up/down- regulation ratio was obtained by measuring WT cells against MK5 activated cells. See material and methods for technical information as well as the data usage policy.

Proteome Analysis

Below the proteome analysis is presented. The analysis methods are presented in the paper Propagating Differential Gene Expression in Protein Interaction Networks. If you are interested in the most reliable results choose the

  • value_type as 'ranked' since this provides a very robust analysis method.
  • intercation map as 'high confidence'
  • network_comparison type as 'Divided' since this leads to a natural grouping of similar proteins
  • filtered as 'true' since this will only use the microarray measurements that have a sufficiently large confidence level.

  • Navigation/Query Panel:
    Click on the attribute name to hide/unhide it. The green arrows can be used to shift columns left/right. Exact word match is written as =..., regular expressions can be matched with ~.... To select all values larger or equal than use >.... This would be <... for values smaller or equal than. To select all values within a specific range use [...,...].
    Filtered green Value Type description Rank Gene Interaction Map network_comparison red Network Comparison Type
    Results: HTML CSV LaTeX Showing element 3180 to 3229 of 14920 in total
    Filtered	1
    green 0.00001
    Value Type Squared
    Interaction Map High confidence
    network_comparison 1
    red 0.00001
    Network Comparison Type Divided
    description Rank nadh-ubiquinone oxidoreductase 24 kda subunit, mitochondrial precursor (ec 1.6.5.3) (ec 1.6.99.3). [source:swissprot;acc:p19404] 2795 nadh-ubiquinone oxidoreductase 30 kda subunit, mitochondrial precursor (ec 1.6.5.3) (ec 1.6.99.3) (complex i-30kd) (ci-30kd). [source:swissprot;acc:o75489] 3081 nadh-ubiquinone oxidoreductase 39 kda subunit, mitochondrial precursor (ec 1.6.5.3) (ec 1.6.99.3) (complex i-39kd) (ci-39kd). [source:swissprot;acc:q16795] 2954 nadh-ubiquinone oxidoreductase 49 kda subunit, mitochondrial precursor (ec 1.6.5.3) (ec 1.6.99.3) (complex i-49kd) (ci-49kd). [source:swissprot;acc:o75306] 2878 nanos (fragment). [source:sptrembl;acc:q8wy41] 2803 neuromedin u receptor 2. [source:refseq;acc:nm_020167] 2991 ngd5 protein homolog (cgi-53). [source:swissprot;acc:q9y366] 3133 nipsnap1 protein. [source:swissprot;acc:q9bpw8] 2770 nipsnap2 protein (glioblastoma amplified sequence). [source:swissprot;acc:o75323] 2918 nk6 transcription factor related, locus 2; nk homeobox family 6, b; glial and testis-specific homeobox protein; homeobox 6b. [source:refseq;acc:nm_177400] 2909 nov protein homolog precursor (novh) (nephroblastoma overexpressed gene protein homolog). [source:swissprot;acc:p48745] 2970 nuclear autoantigen sp-100 (speckled 100 kda) (nuclear dot-associated sp100 protein) (lysp100b). [source:swissprot;acc:p23497] 3184 nuclear factor related to kappa b binding protein. [source:refseq;acc:nm_006165] 2823 nuclear pore complex protein nup214 (nucleoporin nup214) (214 kda nucleoporin) (can protein). [source:swissprot;acc:p35658] 3008 nuclear transport factor 2 (ntf-2) (placental protein 15) (pp15). [source:swissprot;acc:p13662] 3113 nucleolar phosphoprotein p130 (nucleolar 130 kda protein) (140 kda nucleolar phosphoprotein) (nopp140) (nucleolar and coiled-body phosphoprotein 1). [source:swissprot;acc:q14978] 2842 nucleophosmin (npm) (nucleolar phosphoprotein b23) (numatrin) (nucleolar protein no38). [source:swissprot;acc:p06748] 2785 nucleoplasmin 3. [source:swissprot;acc:o75607] 3096 nucleoporin 50 kda (nuclear pore-associated protein 60 kda-like) (pro1146). [source:swissprot;acc:q9ukx7] 3154 nucleosome assembly protein 1-like 1 (nap-1 related protein). [source:swissprot;acc:p55209] 2757 nucleosome assembly protein 1-like 2 (brain-specific protein, x- linked). [source:swissprot;acc:q9ulw6] 2758 nucleosome assembly protein 1-like 4 (nucleosome assembly protein 2) (nap2). [source:swissprot;acc:q99733] 3080 origin recognition complex subunit 2. [source:swissprot;acc:q13416] 3047 origin recognition complex subunit 5. [source:swissprot;acc:o43913] 2851 orphan nuclear receptor nr5a2 (alpha-1-fetoprotein transcription factor) (hepatocytic transcription factor) (b1-binding factor) (hb1f) (cyp7a promoter binding factor). [source:swissprot;acc:o00482] 3042 pak1 interacting protein 1; pak1-interacting protein; pak/plc-interacting protein 1. [source:refseq;acc:nm_017906] 3072 pantothenate kinase 4 (ec 2.7.1.33) (pantothenic acid kinase 4) (hpank4). [source:swissprot;acc:q9nve7] 2883 peptide deformylase, mitochondrial precursor (ec 3.5.1.88) (pdf) (polypeptide deformylase). [source:swissprot;acc:q9hbh1] 2884 peregrin (bromodomain and phd finger-containing protein 1) (br140 protein). [source:swissprot;acc:p55201] 2820 pericentrin 1; frount; nucleoporin nup75. [source:refseq;acc:nm_024844] 3015 phosphate carrier protein, mitochondrial precursor (ptp). [source:swissprot;acc:q00325] 3176 phosphatidylethanolamine-binding protein (pebp) (prostatic binding protein) (hcnppp) (neuropolypeptide h3) (raf kinase inhibitor protein) (rkip) [contains: hippocampal cholinergic neurostimulating peptide (hcnp)]. [source:swissprot;acc:p30086] 3162 phosphatidylinositol glycan class t precursor. [source:refseq;acc:nm_015937] 3024 phosphofurin acidic cluster sorting protein 1 isoform 1; cytosolic sorting protein pacs-1. [source:refseq;acc:nm_018026] 2805 phosphoinositide-3-kinase, class 3; vps34; phosphatidylinositol 3-kinase, class 3. [source:refseq;acc:nm_002647] 3173 phosphoribosyl pyrophosphate synthetase-associated protein 1 (prpp synthetase-associated protein 1) (39 kda phosphoribosypyrophosphate synthetase-associated protein) (pap39). [source:swissprot;acc:q14558] 2869 phosphoribosyl pyrophosphate synthetase-associated protein 2 (prpp synthetase-associated protein 2) (41 kda phosphoribosypyrophosphate synthetase-associated protein) (pap41). [source:swissprot;acc:o60256] 2943 placenta growth factor precursor (plgf). [source:swissprot;acc:p49763] 3036 placental protein 11 precursor (ec 3.4.21.-) (pp11). [source:swissprot;acc:p21128] 3076 platelet-activating factor acetylhydrolase ib alpha subunit (ec 3.1.1.47) (paf acetylhydrolase 45 kda subunit) (paf-ah 45 kda subunit) (paf-ah alpha) (pafah alpha) (lissencephaly-1 protein) (lis- 1). [source:swissprot;acc:p43034] 3221 pogo transposable element with znf domain isoform 1. [source:refseq;acc:nm_015100] 2932 polyhomeotic like 3; early development regulator 3; polyhomeotic 3. [source:refseq;acc:nm_024947] 2809 postsynaptic protein cript; hspc139 protein. [source:refseq;acc:nm_014171] 3034 potassium channel tetramerisation domain containing 3; ny-ren-45 antigen. [source:refseq;acc:nm_016121] 2975 prefoldin subunit 4 (protein c-1). [source:swissprot;acc:q9nqp4] 3130 presenilin enhancer 2; hematopoietic stem/progenitor cells protein mds033. [source:refseq;acc:nm_172341] 3100 prkca-binding protein (protein kinase c-alpha binding protein) (protein interacting with c kinase 1). [source:swissprot;acc:q9nrd5] 3143 programmed cell death 10; apoptosis-related protein 15. [source:refseq;acc:nm_007217] 3052 prostate tumor overexpressed gene 1; prostate tumor over expressed gene 1. [source:refseq;acc:nm_017432] 3106 protease inhibitor 15 preproprotein; 25 kda trypsin inhibitor. [source:refseq;acc:nm_015886] 2966 Legend: - Rank is the rank after comparing the two networks - Value Type describes how the microarray measurement was processed. Ranked indicates that we sorted all measurements and use the rank as red and green value. Measured is the normal measurement. Squared and Rooted are the square and square root respecitcely. This might be valueable to compensate for non linear light distributions. - Network Comparison Type describes whether we divided the red and green types or whether we sutractcted them - Interaction Map specifies whether we used only the hig hconfidence protein-protein interactions or also included the low confidence interactions. - Filtered specifies whether we only included the high confidence microarry measurements or not.

    - http://analysis.yellowcouch.org/