Scientific

MK5 Microarray Data

Affected Genes - Ontology Breakdown - Gene Enrichment - Proteome Analysis

These are the micro-array results of a differential gene expression microarray experiments and the subsequent analysis steps performed on them. The up/down- regulation ratio was obtained by measuring WT cells against MK5 activated cells. See material and methods for technical information as well as the data usage policy.

Proteome Analysis

Below the proteome analysis is presented. The analysis methods are presented in the paper Propagating Differential Gene Expression in Protein Interaction Networks. If you are interested in the most reliable results choose the

  • value_type as 'ranked' since this provides a very robust analysis method.
  • intercation map as 'high confidence'
  • network_comparison type as 'Divided' since this leads to a natural grouping of similar proteins
  • filtered as 'true' since this will only use the microarray measurements that have a sufficiently large confidence level.

    • Navigation/Query Panel:
    Click on the attribute name to hide/unhide it. The green arrows can be used to shift columns left/right. Exact word match is written as =..., regular expressions can be matched with ~.... To select all values larger or equal than use >.... This would be <... for values smaller or equal than. To select all values within a specific range use [...,...].
    Rank Gene Value Type Filtered red green description Hugo network_comparison Interaction Map Network Comparison Type
    Results: HTML CSV LaTeX Showing element 501 to 550 of 3228 in total
    Value Type  : Ranked
    Filtered  : 1
    Interaction Map  : High confidence
    Network Comparison Type  : Divided
    Rank
    		red
    		green
    		description
    		Hugo
    		network_comparison
    501 238.704 255.68 cytochrome c1, heme protein, mitochondrial precursor (cytochrome c-1). [swissprot;acc:p08574] CYC1 1.07112
    502 oligophrenin-1 like protein (gtpase regulator associated with focal adhesion kinase). [swissprot;acc:q9una1] ARHGAP26
    503 senescence marker protein-30 (smp-30) (regucalcin) (rc). [swissprot;acc:q15493] RGN
    504 oligophrenin 1. [swissprot;acc:o60890] OPHN1
    505 251.334 234.672 ad24 protein. [refseq;acc:nm_022451] NOC3L 1.071
    506 224.535 240.476 inhibitor of growth family, member 1; inhibitor of growth 1. [refseq;acc:nm_005537] ING1
    507 251.299 234.729 wd-repeat protein 12 (ytm1 homolog). [swissprot;acc:q9gzl7] WDR12 1.07059
    508 177.698 190.24 mast cell carboxypeptidase a precursor (ec 3.4.17.1) (mc-cpa) (carboxypeptidase a3). [swissprot;acc:p15088] CPA3 1.07058
    509 190.239 carboxypeptidase b precursor. [refseq;acc:nm_020361] CPA6 1.07057
    510 177.699 190.24 plasma carboxypeptidase b2 isoform a preproprotein; carboxypeptidase u; thrombin-activatable fibrinolysis inhibitor; carboxypeptidase b-like protein; thrombin-activable fibrinolysis inhibitor. [refseq;acc:nm_001872] CPB2
    511 250.053 233.725 proliferating-cell nucleolar antigen p120 (proliferation-associated nucleolar protein p120). [swissprot;acc:p46087] NOL1 1.06986
    512 250.794 234.433 block of proliferation 1. [swissprot;acc:q14137] BOP1 1.06979
    513 218.596 204.344 segment polarity protein dishevelled homolog dvl-1 (dishevelled-1) (dsh homolog 1). [swissprot;acc:o14640] DVL1 1.06975
    514 218.613 204.382 segment polarity protein dishevelled homolog dvl-2 (dishevelled-2) (dsh homolog 2). [swissprot;acc:o14641] DVL2 1.06963
    515 251.297 234.941 pescadillo homolog 1. [swissprot;acc:o00541] PES1 1.06962
    516 228.255 213.422 u6 snrna-associated sm-like protein lsm8. [swissprot;acc:o95777] LSM8 1.0695
    517 219.537 234.793 metaxin 2. [swissprot;acc:o75431] MTX2 1.06949
    518 250.858 234.563 nucleostemin; putative nucleotide binding protein, estradiol-induced. [refseq;acc:nm_014366] GNL3 1.06947
    519 218.702 204.582 segment polarity protein dishevelled homolog dvl-3 (dishevelled-3) (dsh homolog 3). [swissprot;acc:q92997] DVL3 1.06902
    520 214.754 229.565 sqv-7-like protein (fragment). [sptrembl;acc:o95454] SLC35D2 1.06897
    521 215.237 230.07 exosome complex exonuclease rrp41 (ec 3.1.13.-) (ribosomal rna processing protein 41) (p12a). [swissprot;acc:q9npd3] EXOSC4 1.06891
    522 222.893 208.523 pleiotropic regulator 1 (prl1homolog, arabidopsis); pleiotropic regulator 1 (prl1, arabidopsis homolog). [refseq;acc:nm_002669] PLRG1
    523 215.237 230.07 exosome complex exonuclease rrp4 (ec 3.1.13.-) (ribosomal rna processing protein 4). [swissprot;acc:q13868] EXOSC2
    524 exosome complex exonuclease rrp40 (ec 3.1.13.-) (ribosomal rna processing protein 40) (p10) (cgi-102). [swissprot;acc:q9nqt5] EXOSC3
    525 exosome complex exonuclease rrp46 (ec 3.1.13.-) (ribosomal rna processing protein 46) (p12b) (chronic myelogenous leukemia tumor antigen 28). [swissprot;acc:q9nqt4] EXOSC5
    526 250.538 234.401 nnp-1 protein (novel nuclear protein 1) (nucleolar protein nop52) (nop52) (d21s2056e). [swissprot;acc:p56182] RRP1 1.06884
    527 248.56 232.579 dead (asp-glu-ala-asp) box polypeptide 31 isoform 1; dead/dexh helicase ddx31. [refseq;acc:nm_022779] DDX31 1.06871
    528 222.146 237.393 yme1-like 1 isoform 3; atp-dependent metalloprotease ftsh1 homolog. [refseq;acc:nm_014263] YME1L1 1.06864
    529 251.653 235.506 atp-dependent rna helicase ddx18 (dead-box protein 18) (myc-regulated dead-box protein) (mrdb). [swissprot;acc:q9nvp1] DDX18 1.06856
    530 227.483 212.994 u6 snrna-associated sm-like protein lsm6 (sm protein f). [swissprot;acc:q9y4y8] LSM6 1.06803
    531 223.356 209.149 nuclear matrix protein nmp200 related to splicing factor prp19. [refseq;acc:nm_014502] PRPF19 1.06793
    532 250.942 235.004 zinc finger-like protein 9. [refseq;acc:nm_033414] ZNF622 1.06782
    533 membrane-associated transporter protein (aim-1 protein) (melanoma antigen aim1). [swissprot;acc:q9umx9] SLC45A2
    534 guanine nucleotide-binding protein-like 1 (gtp-binding protein hsr1). [swissprot;acc:p36915] no value
    535 236.662 252.708 dna-directed rna polymerases i, ii, and iii 17.1 kda polypeptide (ec 2.7.7.6) (rpb17) (rpb8) (rpabc3). [swissprot;acc:p52434] POLR2H 1.0678
    536 242.531 227.136 lupus la protein (sjogren syndrome type b antigen) (ss-b) (la ribonucleoprotein) (la autoantigen). [swissprot;acc:p05455] SSB 1.06778
    537 229.803 215.301 putative pre-mrna splicing factor rna helicase (deah box protein 15) (atp-dependent rna helicase #46). [swissprot;acc:o43143] DHX15 1.06736
    538 237.627 253.604 dna-directed rna polymerase ii 7.6 kda polypeptide (ec 2.7.7.6) (rpb10) (rpb7.6) (rpabc5). [swissprot;acc:p52436] POLR2L 1.06724
    539 236.964 252.86 protein cgi-27 (c21orf19-like protein). [swissprot;acc:q9y316] MEMO1P 1.06708
    540 222.156 208.201 selenide,water dikinase 2 (ec 2.7.9.3) (selenophosphate synthetase 2) (selenium donor protein 2). [swissprot;acc:q99611] SEPHS2 1.06703
    541 flavoprotein oxidoreductase mical2. [refseq;acc:nm_014632] MICAL2
    542 inositol polyphosphate-4-phosphatase, type ii, 105kd; inositol polyphosphate 4-phosphatase ii; 4-phosphatase ii. [refseq;acc:nm_003866] INPP4B
    543 selenide,water dikinase 1 (ec 2.7.9.3) (selenophosphate synthetase 1) (selenium donor protein 1). [swissprot;acc:p49903] SEPHS1
    544 inositol polyphosphate-4-phosphatase, type 1 isoform b; inositol polyphosphate-4-phosphatase, type i, 107kd; 4-phosphatase i; inositol polyphosphate-4-phosphatase i. [refseq;acc:nm_001566] INPP4A
    545 248.866 233.287 eukaryotic translation initiation factor 6 (eif-6) (b4 integrin interactor) (cab) (p27(bbp)) (b(2)gcn homolog). [swissprot;acc:p56537] EIF6 1.06678
    546 207.222 194.262 wiskott-aldrich syndrome protein (wasp). [swissprot;acc:p42768] WAS 1.06671
    547 207.26 194.346 proto-oncogene c-crk (p38) (adapter molecule crk). [swissprot;acc:p46108] CRK 1.06645
    548 tyrosine-protein kinase abl2 (ec 2.7.1.112) (tyrosine kinase arg). [swissprot;acc:p42684] ABL2
    549 proto-oncogene tyrosine-protein kinase src (ec 2.7.1.112) (p60-src) (c-src). [swissprot;acc:p12931] no value
    550 tyrosine-protein kinase hck (ec 2.7.1.112) (p59-hck/p60-hck) (hemopoietic cell kinase). [swissprot;acc:p08631] HCK

    Legend:
    - Rank is the rank after comparing the two networks
    - Gene is the ensembl human gene identifier measured by 1 or more probes on the microarray
    - Hugo is the HGNC identifier if it exists
    - Value Type describes how the microarray measurement was processed. Ranked indicates that we sorted all measurements and use the rank as red and green value. Measured is the normal measurement. Squared and Rooted are the square and square root respecitcely. This might be valueable to compensate for non linear light distributions.
    - Network Comparison Type describes whether we divided the red and green types or whether we sutractcted them
    - Interaction Map specifies whether we used only the hig hconfidence protein-protein interactions or also included the low confidence interactions.
    - Filtered specifies whether we only included the high confidence microarry measurements or not.
    - http://analysis.yellowcouch.org/