Scientific

MK5 Microarray Data

Affected Genes - Ontology Breakdown - Gene Enrichment - Proteome Analysis

These are the micro-array results of a differential gene expression microarray experiments and the subsequent analysis steps performed on them. The up/down- regulation ratio was obtained by measuring WT cells against MK5 activated cells. See material and methods for technical information as well as the data usage policy.

MK5 Proteome Analysis

Table 3: Type II network, divided ranks. This table presents the output of the simulation of a type-II network in which we relied on the high confidence interactions and micro-array results. The output of the micro-array was then ranked and these ranks used as input into the network simulation. After performing two such simulations (one for the MK5 off and one for the MK5 on microarray output), we divided the ranks and sorted the results accordingly. The output of this technique is a collection of proteins that cluster naturally together.


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Gene Value Type Rank description Network Comparison Type Interaction Map green Filtered red network_comparison
Results: HTML CSV LaTeX Showing element 1563 to 1612 of 7460 in total
Value Type  : Ranked
Interaction Map  : High confidence
Rank
description
Network Comparison Type
green
Filtered
red
network_comparison
391 spliceosomal u5 snrnp-specific 15 kda protein (dim1 protein homolog) (thioredoxin-like u5 snrnp protein u5-15kd). [swissprot;acc:o14834] Subtracted 207.072 1 225.719 18.647
testis-specific chromodomain protein y protein 2. [swissprot;acc:q9y6f7] 10686.7 0 10302.7 384
392 60s ribosomal protein l13a (23 kda highly basic protein). [swissprot;acc:p40429] Divided 263.615 1 241.83 1.09008
polymerase (rna) iii (dna directed) (62kd). [refseq;acc:nm_006468] Subtracted 257.705 239.092 18.613
seven in absentia homolog 1. [refseq;acc:nm_003031] Divided 10201.4 0 9854.67 1.03518
testis-specific chromodomain y protein 1. [swissprot;acc:q9y6f8] Subtracted 10687.4 10303.7 383.7
393 dna-directed rna polymerases iii 39 kda polypeptide (ec 2.7.7.6) (rna polymerase iii c39 subunit). [swissprot;acc:q9h1d9] 257.327 1 238.921 18.406
spliceosomal u5 snrnp-specific 15 kda protein (dim1 protein homolog) (thioredoxin-like u5 snrnp protein u5-15kd). [swissprot;acc:o14834] Divided 207.072 225.719 1.09005
transcription elongation factor a protein 1 (transcription elongation factor s-ii protein 1) (transcription elongation factor tfiis.o). [swissprot;acc:p23193] 13010 0 12576 1.03451
williams-beuren syndrome chromosome region 14 protein (ws basic-helix- loop-helix leucine zipper protein) (ws-bhlh) (mlx interactor). [swissprot;acc:q9np71] Subtracted 5921 5540 381
394 60s ribosomal protein l12. [swissprot;acc:p30050] Divided 9857.79 10196.9 1.0344
clathrin heavy chain 1 (clh-17). [swissprot;acc:q00610] Subtracted 226.226 1 207.889 18.337
dipeptidylpeptidase 9; dipeptidyl peptidase 9; dipeptidyl peptidase iv-related protein-2. [refseq;acc:nm_139159] Divided 238.407 218.827 1.08948
translin-associated protein x (translin-associated factor x). [swissprot;acc:q99598] Subtracted 20495 0 20875 380
395 40s ribosomal protein s27 (metallopan-stimulin 1) (mps-1). [swissprot;acc:p42677] 9936.37 9566.15 370.22
dipeptidyl peptidase 8 isoform 2; dipeptidyl peptidase 8. [refseq;acc:nm_017743] Divided 238.407 1 218.827 1.08948
dj999l4.1 (novel protein similar to ribosomal protein l12 (rpl12)). [sptrembl;acc:q9nq02] 9856.7 0 10195.5 1.03437
phosducin-like protein (phlp). [swissprot;acc:q13371] Subtracted 226.218 1 207.908 18.31
396 60s ribosomal protein l12 like protein. [sptrembl;acc:o60886] Divided 9856.12 0 10194.8 1.03436
putative eukaryotic translation initiation factor 1a (eif-1a) (eif- 4c). [swissprot;acc:o75642] 262.34 1 240.801 1.08945
replication protein a 70 kda dna-binding subunit (rp-a) (rf-a) (replication factor-a protein 1) (single-stranded dna-binding protein). [swissprot;acc:p27694] Subtracted 226.218 207.908 18.31
transcription factor e2f2 (e2f-2). [swissprot;acc:q14209] 8139.71 0 7769.96 369.75
397 60s ribosomal protein l10a (csa-19). [swissprot;acc:p53025] Divided 9676.53 10006.9 1.03414
replication protein a 32 kda subunit (rp-a) (rf-a) (replication factor-a protein 2). [swissprot;acc:p15927] Subtracted 226.218 1 207.908 18.31
ribosomal protein s27-like protein; 40s ribosomal protein s27 isoform. [refseq;acc:nm_015920] 9935.19 0 9565.54 369.65
t-complex protein 1, gamma subunit (tcp-1-gamma) (cct-gamma). [swissprot;acc:p49368] Divided 101.613 1 110.689 1.08932
398 glycerol kinase (ec 2.7.1.30) (atp:glycerol 3-phosphotransferase) (glycerokinase) (gk). [swissprot;acc:p32189] 17267.9 0 16699.2 1.03406
muscle-specific beta 1 integrin binding protein. [refseq;acc:nm_014446] 238.358 1 218.818 1.0893
peroxisomal 3,2-trans-enoyl-coa isomerase (ec 5.3.3.8) (dodecenoyl-coa delta-isomerase) (d3,d2-enoyl-coa isomerase) (dbi-related protein 1) (drs-1) (hepatocellular carcinoma-associated antigen 88). [swissprot;acc:o75521] Subtracted 10320.9 0 9953.39 367.51
phosducin (phd) (33 kda phototransducing protein) (meka protein). [swissprot;acc:p20941] 226.218 1 207.908 18.31
399 carboxypeptidase h precursor (ec 3.4.17.10) (cph) (carboxypeptidase e) (cpe) (enkephalin convertase) (prohormone processing carboxypeptidase). [swissprot;acc:p16870] 7311 0 7670 359
clathrin heavy chain 1 (clh-17). [swissprot;acc:q00610] Divided 226.226 1 207.889 1.08821
dna repair protein rad52 homolog. [swissprot;acc:p43351] Subtracted 226.218 207.908 18.31
glycerol kinase, testis specific 1 (ec 2.7.1.30) (atp:glycerol 3- phosphotransferase) (glycerokinase) (gk). [swissprot;acc:q14409] Divided 17271.4 0 16703.1 1.03402
400 clathrin heavy chain 2 (clh-22). [swissprot;acc:p53675] Subtracted 226.209 1 207.931 18.278
glutamine synthetase (ec 6.3.1.2) (glutamate--ammonia ligase). [swissprot;acc:p15104] 12224.2 0 12581.7 357.5
glycerol kinase, testis specific 2 (ec 2.7.1.30) (atp:glycerol 3- phosphotransferase) (glycerokinase) (gk). [swissprot;acc:q14410] Divided 17278.4 16711 1.03395
phosducin-like protein (phlp). [swissprot;acc:q13371] 226.218 1 207.908 1.08807
401 mitochondrial 28s ribosomal protein s6 (mrp-s6). [swissprot;acc:p82932] Subtracted 20558 0 20914 356
mki67 (fha domain) interacting nucleolar phosphoprotein; nucleolar phosphoprotein nopp34; nucleolar protein interacting with the fha domain of pki-67. [refseq;acc:nm_032390] 236.224 1 254.434 18.21
replication protein a 70 kda dna-binding subunit (rp-a) (rf-a) (replication factor-a protein 1) (single-stranded dna-binding protein). [swissprot;acc:p27694] Divided 226.218 207.908 1.08807
transcription factor dp-1 (e2f dimerization partner 1) (drtf1- polypeptide-1) (drtf1). [swissprot;acc:q14186] 11904.9 0 12305.9 1.03368
402 adaptor-related protein complex 1, mu 2 subunit (mu-adaptin 2) (adaptor protein complex ap-1 mu-2 subunit) (golgi adaptor ha1/ap1 adaptin mu-2 subunit) (clathrin assembly protein assembly protein complex 1 medium chain 2) (ap-mu chain family member mu1b). [swissprot;acc:q9y6q5] 10918 10562.4 1.03367
Subtracted 355.6
highly expressed in cancer, rich in leucine heptad repeats. [refseq;acc:nm_006101] 249.983 1 231.802 18.181
replication protein a 32 kda subunit (rp-a) (rf-a) (replication factor-a protein 2). [swissprot;acc:p15927] Divided 226.218 207.908 1.08807
403 microtubule-actin crosslinking factor 1, isoform 4. [swissprot;acc:q96pk2] Subtracted 246.338 228.468 17.87
phosducin (phd) (33 kda phototransducing protein) (meka protein). [swissprot;acc:p20941] Divided 226.218 207.908 1.08807
scribble. [refseq;acc:nm_015356] Subtracted 12719 0 12370.5 348.5
udp-glucuronosyltransferase 2b17 precursor, microsomal (ec 2.4.1.17) (udpgt) (c19-steroid specific udp-glucuronosyltransferase). [swissprot;acc:o75795] Divided 10111.2 9783.63 1.03348

Legend:
- Rank is the rank after comparing the two networks
- Gene is the ensembl human gene identifier measured by 1 or more probes on the microarray
- Value Type describes how the microarray measurement was processed. Ranked indicates that we sorted all measurements and use the rank as red and green value. Measured is the normal measurement. Squared and Rooted are the square and square root respecitcely. This might be valueable to compensate for non linear light distributions.
- Network Comparison Type describes whether we divided the red and green types or whether we sutractcted them
- Interaction Map specifies whether we used only the hig hconfidence protein-protein interactions or also included the low confidence interactions.
- Filtered specifies whether we only included the high confidence microarry measurements or not.
- http://analysis.yellowcouch.org/