Scientific

MK5 Microarray Data

Affected Genes - Ontology Breakdown - Gene Enrichment - Proteome Analysis

These are the micro-array results of a differential gene expression microarray experiments and the subsequent analysis steps performed on them. The up/down- regulation ratio was obtained by measuring WT cells against MK5 activated cells. See material and methods for technical information as well as the data usage policy.

MK5 Proteome Analysis

Table 3: Type II network, divided ranks. This table presents the output of the simulation of a type-II network in which we relied on the high confidence interactions and micro-array results. The output of the micro-array was then ranked and these ranks used as input into the network simulation. After performing two such simulations (one for the MK5 off and one for the MK5 on microarray output), we divided the ranks and sorted the results accordingly. The output of this technique is a collection of proteins that cluster naturally together.

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Rank Gene Hugo description Value Type Network Comparison Type Interaction Map Filtered red green network_comparison

Results: HTML CSV LaTeX

Showing element 33 to 82 of 3228 in total

Value Type	Ranked
Network Comparison Type	Divided
Interaction Map	High confidence
Filtered	1

Rank	Hugo	description	red	green	network_comparison	
33	PPP2R1B	serine/threonine protein phosphatase 2a, 65 kda regulatory subunit a, beta isoform (pp2a, subunit a, pr65-beta isoform) (pp2a, subunit a, r1-beta isoform). [source:swissprot;acc:p30154]	188.688	242.742	1.28647	
34	PPP2R1A	serine/threonine protein phosphatase 2a, 65 kda regulatory subunit a, alpha isoform (pp2a, subunit a, pr65-alpha isoform) (pp2a, subunit a, r1-alpha isoform) (medium tumor antigen-associated 61 kda protein). [source:swissprot;acc:p30153]	189.07	242.453	1.28235	
35	NFYC	nuclear transcription factor y subunit gamma (nf-y protein chain c) (nuclear factor yc) (nf-yc) (ccaat-binding transcription factor subunit c) (cbf-c) (transactivator hsm-1/2). [source:swissprot;acc:q13952]	260.532	204.309	1.27519	
36	NFYA	ccaat-binding transcription factor subunit b (cbf-b) (nf-y protein chain a) (nf-ya) (caat-box dna binding protein subunit a). [source:swissprot;acc:p23511]	260.532	204.309	1.27519	
37	PPP1R14A	protein phosphatase 1, regulatory (inhibitor) subunit 14a; 17-kda pkc-potentiated inhibitory protein of pp1. [source:refseq;acc:nm_033256]	322	253	1.27273	
38	MFAP4	microfibril-associated glycoprotein 4 precursor. [source:swissprot;acc:p55083]	322	253	1.27273	
39	PPP1R14D	protein phosphatase 1, regulatory subunit 14d; pkc-dependent pp1 inhibitory protein. [source:refseq;acc:nm_017726]	322	253	1.27273	
40	FCN2	ficolin 2 precursor (collagen/fibrinogen domain-containing protein 2) (ficolin-b) (ficolin b) (serum lectin p35) (ebp-37) (hucolin) (l- ficolin). [source:swissprot;acc:q15485]	322	253	1.27273	
41		protein phosphatase 1, regulatory (inhibitor) subunit 14c; serologically defined breast cancer antigen ny-br-81; pkc-potentiated pp1 inhibitory protein. [source:refseq;acc:nm_030949]	322	253	1.27273	
42	FOS	proto-oncogene protein c-fos (cellular oncogene fos) (g0/g1 switch regulatory protein 7). [source:swissprot;acc:p01100]	204.641	160.957	1.2714	
43		jun dimerization protein. [source:refseq;acc:nm_130469]	204.641	160.957	1.2714	
44	FOSL2	fos-related antigen 2. [source:swissprot;acc:p15408]	204.641	161.046	1.2707	
45	FOSB	protein fosb (g0/g1 switch regulatory protein 3). [source:swissprot;acc:p53539]	204.641	161.051	1.27066	
46	FOSL1	fos-related antigen 1 (fra-1). [source:swissprot;acc:p15407]	204.642	161.068	1.27053	
47	TMEM132A	gbp protein isoform a. [source:refseq;acc:nm_017870]	325.586	256.492	1.26938	
48	GABARAP	gaba(a) receptor-associated protein; gaba(a)-receptor-associated protein. [source:refseq;acc:nm_007278]	65.4586	83.0461	1.26868	
49	ARRB2	beta-arrestin 2 (arrestin, beta 2). [source:swissprot;acc:p32121]	133	105	1.26667	
50	ARRB1	beta-arrestin 1 (arrestin, beta 1). [source:swissprot;acc:p49407]	133	105	1.26667	
51	SPATA6	spermatogenesis associated 6. [source:refseq;acc:nm_019073]	133	105	1.26667	
52	AMPD2	amp deaminase 2 (ec 3.5.4.6) (amp deaminase isoform l). [source:swissprot;acc:q01433]	133	105	1.26667	
53	JUNB	transcription factor jun-b. [source:swissprot;acc:p17275]	201.144	158.868	1.26611	
54	JUND	transcription factor jun-d. [source:swissprot;acc:p17535]	200.941	158.941	1.26425	
55	PRKRAP1	protein kinase, interferon-inducible double stranded rna dependent activator; protein activator of the interferon-induced protein kinase. [source:refseq;acc:nm_003690]	320.927	254.434	1.26134	
56		guanine nucleotide-binding protein g(i)/g(s)/g(o) gamma-7 subunit. [source:swissprot;acc:o60262]	266.856	336.233	1.25998	
57	GNG12	guanine nucleotide-binding protein g(i)/g(s)/g(o) gamma-12 subunit. [source:swissprot;acc:q9ubi6]	266.856	336.233	1.25998	
58	GNG4	guanine nucleotide-binding protein g(i)/g(s)/g(o) gamma-4 subunit. [source:swissprot;acc:p50150]	266.863	336.191	1.25979	
59	GNG3	guanine nucleotide-binding protein g(i)/g(s)/g(o) gamma-3 subunit. [source:swissprot;acc:p29798]	266.863	336.19	1.25978	
60	GNG8	guanine nucleotide-binding protein g(i)/g(s)/g(o) gamma-8 subunit (gamma-9). [source:swissprot;acc:q9uk08]	266.864	336.184	1.25976	
61	GNG5	guanine nucleotide-binding protein g(i)/g(s)/g(o) gamma-5 subunit. [source:swissprot;acc:p30670]	266.864	336.182	1.25975	
62	GNG10	guanine nucleotide-binding protein g(i)/g(s)/g(o) gamma-10 subunit. [source:swissprot;acc:p50151]	266.865	336.181	1.25974	
63		guanine nucleotide-binding protein g(i)/g(s)/g(o) gamma-5 like subunit. [source:swissprot;acc:q9y3k8]	266.865	336.178	1.25973	
64	GNG11	guanine nucleotide-binding protein g(i)/g(s)/g(o) gamma-11 subunit. [source:swissprot;acc:p50152]	266.868	336.159	1.25965	
65	GNAQ	guanine nucleotide-binding protein g(q), alpha subunit. [source:swissprot;acc:p50148]	267.136	336.077	1.25807	
66	GNA11	guanine nucleotide-binding protein g(y), alpha subunit (alpha-11). [source:swissprot;acc:p29992]	267.136	336.077	1.25807	
67	GNAT2	guanine nucleotide-binding protein g(t), alpha-2 subunit (transducin alpha-2 chain). [source:swissprot;acc:p19087]	267.14	336.075	1.25805	
68	GNAZ	guanine nucleotide-binding protein g(z), alpha subunit (g(x) alpha chain) (gz-alpha). [source:swissprot;acc:p19086]	267.139	336.075	1.25805	
69	GNAT1	guanine nucleotide-binding protein g(t), alpha-1 subunit (transducin alpha-1 chain). [source:swissprot;acc:p11488]	267.14	336.075	1.25805	
70	GNAI2	guanine nucleotide-binding protein g(i), alpha-2 subunit (adenylate cyclase-inhibiting g alpha protein). [source:swissprot;acc:p04899]	267.142	336.074	1.25804	
71	GNAI1	guanine nucleotide-binding protein g(i), alpha-1 subunit (adenylate cyclase-inhibiting g alpha protein). [source:swissprot;acc:p04898]	267.144	336.073	1.25802	
72	GNAI3	guanine nucleotide-binding protein g(k), alpha subunit (g(i) alpha-3). [source:swissprot;acc:p08754]	267.144	336.073	1.25802	
73	JPH3	junctophilin 3 (junctophilin type 3) (jp-3). [source:swissprot;acc:q8wxh2]	288	362	1.25694	
74	JPH2	junctophilin 2 (junctophilin type 2) (jp-2). [source:swissprot;acc:q9br39]	288	362	1.25694	
75	SMPD2	sphingomyelin phosphodiesterase 2 (ec 3.1.4.12) (neutral sphingomyelinase) (nsmase) (n-smase) (lyso-platelet activating factor- phospholipase c) (lyso-paf-plc). [source:swissprot;acc:o60906]	288	362	1.25694	
76	TMEM33	db83 protein. [source:swissprot;acc:p57088]	288	362	1.25694	
77	CACNG2	voltage-dependent calcium channel gamma-2 subunit (neuronal voltage- gated calcium channel gamma-2 subunit). [source:swissprot;acc:q9y698]	254.692	202.668	1.2567	
78	CACNG8	voltage-dependent calcium channel gamma-8 subunit (neuronal voltage- gated calcium channel gamma-8 subunit). [source:swissprot;acc:q8wxs5]	254.693	202.668	1.2567	
79	CACNG3	voltage-dependent calcium channel gamma-3 subunit (neuronal voltage- gated calcium channel gamma-3 subunit). [source:swissprot;acc:o60359]	254.693	202.668	1.2567	
80	CACNG4	voltage-dependent calcium channel gamma-4 subunit (neuronal voltage- gated calcium channel gamma-4 subunit). [source:swissprot;acc:q9ubn1]	254.691	202.668	1.25669	
81	CACNG5	voltage-dependent calcium channel gamma-5 subunit (neuronal voltage- gated calcium channel gamma-5 subunit). [source:swissprot;acc:q9uf02]	254.614	202.646	1.25645	
82	GNB5	guanine nucleotide-binding protein beta subunit 5 (transducin beta chain 5). [source:swissprot;acc:o14775]	267.636	335.359	1.25304	

Legend:
- Rank is the rank after comparing the two networks
- Hugo is the HGNC identifier if it exists
- Value Type describes how the microarray measurement was processed. Ranked indicates that we sorted all measurements and use the rank as red and green value. Measured is the normal measurement. Squared and Rooted are the square and square root respecitcely. This might be valueable to compensate for non linear light distributions.
- Network Comparison Type describes whether we divided the red and green types or whether we sutractcted them
- Interaction Map specifies whether we used only the hig hconfidence protein-protein interactions or also included the low confidence interactions.
- Filtered specifies whether we only included the high confidence microarry measurements or not.

- http://analysis.yellowcouch.org/