Scientific

MK5 Microarray Data


Affected Genes - Ontology Breakdown - Gene Enrichment - Proteome Analysis

These are the micro-array results of a differential gene expression microarray experiments and the subsequent analysis steps performed on them. The up/down- regulation ratio was obtained by measuring WT cells against MK5 activated cells. See material and methods for technical information as well as the data usage policy.

MK5 Proteome Analysis

Table 3: Type II network, divided ranks. This table presents the output of the simulation of a type-II network in which we relied on the high confidence interactions and micro-array results. The output of the micro-array was then ranked and these ranks used as input into the network simulation. After performing two such simulations (one for the MK5 off and one for the MK5 on microarray output), we divided the ranks and sorted the results accordingly. The output of this technique is a collection of proteins that cluster naturally together.


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Rank Gene description Value Type Network Comparison Type Interaction Map Filtered red green network_comparison
Results: HTML CSV LaTeX Showing element 1651 to 1700 of 3228 in total
Value Type: Measured
Network Comparison Type: Divided
Interaction Map: High confidence
Filtered: 1
\def\wcA{0.16666666666667\textwidth} \def\wcB{0.16666666666667\textwidth} \def\wcC{0.16666666666667\textwidth} \def\wcD{0.16666666666667\textwidth} \def\wcE{0.16666666666667\textwidth} \begin{longtable}{|c|c|c|c|c|} \hline \parbox{\wcA}{\vspace{3pt}\noindent Rank\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent description\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent red\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent green\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent network\_comparison\vspace{3pt}}\\ \hline \hline \parbox{\wcA}{\vspace{3pt}\noindent 1651\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent cbf1 interacting corepressor. [source:refseq;acc:nm\_004882]\vspace{3pt}}&\multirow{2}{*}{\parbox{\wcC}{\vspace{3pt}\noindent 5944.24\vspace{3pt}}}&\multirow{2}{*}{\parbox{\wcD}{\vspace{3pt}\noindent 6358.1\vspace{3pt}}}&\multirow{2}{*}{\parbox{\wcE}{\vspace{3pt}\noindent 1.06962\vspace{3pt}}}\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1652\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent evolutionarily conserved g-patch domain containing. [source:refseq;acc:nm\_018025]\vspace{3pt}}&&&\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 1653\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent transcription factor mafg (v-maf musculoaponeurotic fibrosarcoma oncogene homolog g) (hmaf). [source:swissprot;acc:o15525]\vspace{3pt}}&\multirow{2}{*}{\parbox{\wcC}{\vspace{3pt}\noindent 7433.73\vspace{3pt}}}&\multirow{2}{*}{\parbox{\wcD}{\vspace{3pt}\noindent 7950.61\vspace{3pt}}}&\multirow{12}{*}{\parbox{\wcE}{\vspace{3pt}\noindent 1.06953\vspace{3pt}}}\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1654\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent transcription factor maff (v-maf musculoaponeurotic fibrosarcoma oncogene homolog f) (u-maf). [source:swissprot;acc:q9ulx9]\vspace{3pt}}&&&\\\cline{1-4} \parbox{\wcA}{\vspace{3pt}\noindent 1655\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 40s ribosomal protein s26. [source:swissprot;acc:p02383]\vspace{3pt}}&\multirow{3}{*}{\parbox{\wcC}{\vspace{3pt}\noindent 5488.7\vspace{3pt}}}&\multirow{3}{*}{\parbox{\wcD}{\vspace{3pt}\noindent 5131.89\vspace{3pt}}}&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1656\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent mitochondrial import inner membrane translocase subunit tim22. [source:swissprot;acc:q9y584]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1657\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent atp synthase mitochondrial f1 complex assembly factor 2. [source:refseq;acc:nm\_145691]\vspace{3pt}}&&&\\\cline{1-4} \parbox{\wcA}{\vspace{3pt}\noindent 1658\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent transcription factor mafk (erythroid transcription factor nf-e2 p18 subunit). [source:swissprot;acc:o60675]\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 7433.73\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent 7950.61\vspace{3pt}}&\\\cline{1-4} \parbox{\wcA}{\vspace{3pt}\noindent 1659\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent ubiquinol-cytochrome c reductase complex 14 kda protein (ec 1.10.2.2) (complex iii subunit vi) (qp-c). [source:swissprot;acc:p14927]\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 5488.7\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent 5131.89\vspace{3pt}}&\\\cline{1-4} \parbox{\wcA}{\vspace{3pt}\noindent 1660\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent cell adhesion molecule with homology to l1cam precursor; neural cell adhesion molecule; cell adhesion molecule l1-like; l1 cell adhesion molecule 2. [source:refseq;acc:nm\_006614]\vspace{3pt}}&\multirow{5}{*}{\parbox{\wcC}{\vspace{3pt}\noindent 7433.73\vspace{3pt}}}&\multirow{5}{*}{\parbox{\wcD}{\vspace{3pt}\noindent 7950.61\vspace{3pt}}}&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1661\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent neural cell adhesion molecule l1 precursor (n-cam l1) (cd171 antigen). [source:swissprot;acc:p32004]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1662\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent transcription initiation factor tfiid 31 kda subunit (tafii-31) (tafii-32) (tafii32). [source:swissprot;acc:q16594]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1663\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent nuclear factor erythroid 2 related factor 1 (nf-e2 related factor 1) (nfe2-related factor 1) (nuclear factor, erythroid derived 2, like 1) (transcription factor 11) (transcription factor hbz17) (transcription factor lcr-f1) (locus control region-factor 1). [source:swissprot;acc:q14494]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1664\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent nuclear factor (erythroid-derived 2)-like 3; nf-e2-related factor 3. [source:refseq;acc:nm\_004289]\vspace{3pt}}&&&\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 1665\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent fatty acid-binding protein, adipocyte (afabp) (adipocyte lipid-binding protein) (albp) (a-fabp). [source:swissprot;acc:p15090]\vspace{3pt}}&\multirow{12}{*}{\parbox{\wcC}{\vspace{3pt}\noindent 5791.14\vspace{3pt}}}&\multirow{12}{*}{\parbox{\wcD}{\vspace{3pt}\noindent 6193.02\vspace{3pt}}}&\multirow{12}{*}{\parbox{\wcE}{\vspace{3pt}\noindent 1.0694\vspace{3pt}}}\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1666\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent fatty acid-binding protein, epidermal (e-fabp) (psoriasis-associated fatty acid-binding protein homolog) (pa-fabp). [source:swissprot;acc:q01469]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1667\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent fatty acid-binding protein, brain (b-fabp) (brain lipid-binding protein) (blbp) (mammary derived growth inhibitor related). [source:swissprot;acc:o15540]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1668\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent wd-repeat protein 4. [source:swissprot;acc:p57081]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1669\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent myelin p2 protein. [source:swissprot;acc:p02689]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1670\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent kelch-like protein 8. [source:swissprot;acc:q9p2g9]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1671\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent phenylethanolamine n-methyltransferase (ec 2.1.1.28) (pnmtase) (noradrenaline n-methyltransferase). [source:swissprot;acc:p11086]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1672\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent beta-carotene dioxygenase 2; putative b,b-carotene-9',10'-dioxygenase. [source:refseq;acc:nm\_031938]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1673\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent beta-carotene 15, 15'-dioxygenase; beta-carotene 15,15'-monooxygenase. [source:refseq;acc:nm\_017429]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1674\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent fatty acid-binding protein, heart (h-fabp) (muscle fatty acid-binding protein) (m-fabp) (mammary-derived growth inhibitor) (mdgi). [source:swissprot;acc:p05413]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1675\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent retinal pigment epithelium-specific protein 65kda; retinal pigment epithelium-specific protein (65kd); retinitis pigmentosa 20 (autosomal recessive). [source:refseq;acc:nm\_000329]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1676\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent indolethylamine n-methyltransferase (ec 2.1.1.49) (aromatic alkylamine n-methyltransferase) (indolamine n-methyltransferase) (arylamine n- methyltransferase) (amine n-methyltransferase). [source:swissprot;acc:o95050]\vspace{3pt}}&&&\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 1677\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent ribonuclease hi large subunit (ec 3.1.26.-) (rnase hi large subunit) (rnase h(35)) (ribonuclease h2) (rnase h2). [source:swissprot;acc:o75792]\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 6075.19\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent 6496.61\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 1.06937\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 1678\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent cleavage and polyadenylation specific factor 5, 25 kd subunit; pre-mrna cleavage factor im (25kd); pre-mrna cleavage factor im, 25kd subunit. [source:refseq;acc:nm\_007006]\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 5763.25\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent 6162.98\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 1.06936\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 1679\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent proteasome subunit beta type 3 (ec 3.4.25.1) (proteasome theta chain) (proteasome chain 13) (proteasome component c10-ii). [source:swissprot;acc:p49720]\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 6180.24\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent 6608.11\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 1.06923\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 1680\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent methylenetetrahydrofolate reductase (ec 1.5.1.20). [source:swissprot;acc:p42898]\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 6436.73\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent 6882.16\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 1.0692\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 1681\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent f-actin capping protein alpha-3 subunit (capz alpha-3) (germ cell- specific protein 3). [source:swissprot;acc:q96kx2]\vspace{3pt}}&\multirow{2}{*}{\parbox{\wcC}{\vspace{3pt}\noindent 6304.74\vspace{3pt}}}&\multirow{2}{*}{\parbox{\wcD}{\vspace{3pt}\noindent 6740.66\vspace{3pt}}}&\multirow{2}{*}{\parbox{\wcE}{\vspace{3pt}\noindent 1.06914\vspace{3pt}}}\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1682\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent f-actin capping protein beta subunit (capz beta). [source:swissprot;acc:p47756]\vspace{3pt}}&&&\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 1683\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent ring finger protein 20. [source:refseq;acc:nm\_019592]\vspace{3pt}}&\multirow{2}{*}{\parbox{\wcC}{\vspace{3pt}\noindent 5419.54\vspace{3pt}}}&\multirow{2}{*}{\parbox{\wcD}{\vspace{3pt}\noindent 5793.94\vspace{3pt}}}&\multirow{2}{*}{\parbox{\wcE}{\vspace{3pt}\noindent 1.06908\vspace{3pt}}}\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1684\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent ring finger protein 40; 95 kda retinoblastoma protein binding protein; likely ortholog of rat staring. [source:refseq;acc:nm\_014771]\vspace{3pt}}&&&\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 1685\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent gc-rich sequence dna-binding factor homolog. [source:swissprot;acc:q9y5b6]\vspace{3pt}}&\multirow{2}{*}{\parbox{\wcC}{\vspace{3pt}\noindent 6088.07\vspace{3pt}}}&\multirow{2}{*}{\parbox{\wcD}{\vspace{3pt}\noindent 6508.45\vspace{3pt}}}&\multirow{2}{*}{\parbox{\wcE}{\vspace{3pt}\noindent 1.06905\vspace{3pt}}}\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1686\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent toll-like receptor 2 precursor (toll/interleukin 1 receptor-like protein 4). [source:swissprot;acc:o60603]\vspace{3pt}}&&&\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 1687\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent serpin b12. [source:swissprot;acc:q96p63]\vspace{3pt}}&\multirow{9}{*}{\parbox{\wcC}{\vspace{3pt}\noindent 5767.69\vspace{3pt}}}&\multirow{9}{*}{\parbox{\wcD}{\vspace{3pt}\noindent 6165.87\vspace{3pt}}}&\multirow{9}{*}{\parbox{\wcE}{\vspace{3pt}\noindent 1.06904\vspace{3pt}}}\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1688\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent megsin (tp55) (serpin b7). [source:swissprot;acc:o75635]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1689\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent hurpin (hacat uv-repressible serpin) (protease inhibitor 13) (headpin) (serpin b13). [source:swissprot;acc:q9uiv8]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1690\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent oxysterols receptor lxr-beta (liver x receptor beta) (nuclear orphan receptor lxr-beta) (ubiquitously-expressed nuclear receptor) (nuclear receptor ner). [source:swissprot;acc:p55055]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1691\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent upf0183 protein. [source:swissprot;acc:q9bsu1]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1692\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent antithrombin-iii precursor (atiii) (pro0309). [source:swissprot;acc:p01008]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1693\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent translation initiation factor eif-2b alpha subunit (eif-2b gdp-gtp exchange factor). [source:swissprot;acc:q14232]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1694\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent squamous cell carcinoma antigen 1 (scca-1) (protein t4-a). [source:swissprot;acc:p29508]\vspace{3pt}}&&&\\\cline{1-2} \parbox{\wcA}{\vspace{3pt}\noindent 1695\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent oxysterols receptor lxr-alpha (liver x receptor alpha) (nuclear orphan receptor lxr-alpha). [source:swissprot;acc:q13133]\vspace{3pt}}&&&\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 1696\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent wiskott-aldrich syndrome protein family member 2 (wasp-family protein member 2) (verprolin homology domain-containing protein 2). [source:swissprot;acc:q9y6w5]\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 6304.22\vspace{3pt}}&\multirow{4}{*}{\parbox{\wcD}{\vspace{3pt}\noindent 6738.77\vspace{3pt}}}&\multirow{4}{*}{\parbox{\wcE}{\vspace{3pt}\noindent 1.06893\vspace{3pt}}}\\\cline{1-3} \parbox{\wcA}{\vspace{3pt}\noindent 1697\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent wiskott-aldrich syndrome protein family member 3 (wasp-family protein member 3) (verprolin homology domain-containing protein 3). [source:swissprot;acc:q9upy6]\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 6304.21\vspace{3pt}}&&\\\cline{1-3} \parbox{\wcA}{\vspace{3pt}\noindent 1698\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent wiskott-aldrich syndrome protein family member 1 (wasp-family protein member 1) (verprolin homology domain-containing protein 1). [source:swissprot;acc:q92558]\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 6304.22\vspace{3pt}}&&\\\cline{1-3} \parbox{\wcA}{\vspace{3pt}\noindent 1699\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent rest corepressor. [source:refseq;acc:nm\_015156]\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 6304.21\vspace{3pt}}&&\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 1700\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent phosphatidylinositol-4-phosphate 3-kinase c2 domain-containing gamma polypeptide (ec 2.7.1.154) (phosphoinositide 3-kinase-c2-gamma) (ptdins-3-kinase c2 gamma) (pi3k-c2gamma). [source:swissprot;acc:o75747]\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 6326.24\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent 6762.27\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 1.06892\vspace{3pt}}\\\hline nd{longtable}
Legend:
- Rank is the rank after comparing the two networks
- Gene is the ensembl human gene identifier measured by 1 or more probes on the microarray
- Value Type describes how the microarray measurement was processed. Ranked indicates that we sorted all measurements and use the rank as red and green value. Measured is the normal measurement. Squared and Rooted are the square and square root respecitcely. This might be valueable to compensate for non linear light distributions.
- Network Comparison Type describes whether we divided the red and green types or whether we sutractcted them
- Interaction Map specifies whether we used only the hig hconfidence protein-protein interactions or also included the low confidence interactions.
- Filtered specifies whether we only included the high confidence microarry measurements or not.

- http://analysis.yellowcouch.org/