Scientific

MK5 Microarray Data

Affected Genes - Ontology Breakdown - Gene Enrichment - Proteome Analysis

These are the micro-array results of a differential gene expression microarray experiments and the subsequent analysis steps performed on them. The up/down- regulation ratio was obtained by measuring WT cells against MK5 activated cells. See material and methods for technical information as well as the data usage policy.

MK5 Proteome Analysis

Table 3: Type II network, divided ranks. This table presents the output of the simulation of a type-II network in which we relied on the high confidence interactions and micro-array results. The output of the micro-array was then ranked and these ranks used as input into the network simulation. After performing two such simulations (one for the MK5 off and one for the MK5 on microarray output), we divided the ranks and sorted the results accordingly. The output of this technique is a collection of proteins that cluster naturally together.

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Rank Gene description Value Type Network Comparison Type Interaction Map Filtered red green network_comparison

Results: HTML CSV LaTeX

Showing element 773 to 822 of 12912 in total

Network Comparison Type	Divided
Interaction Map	High confidence
Filtered	1

Rank	description	Value Type	red	green	network_comparison	
194	aquaporin-chip (water channel protein for red blood cells and kidney proximal tubule) (aquaporin 1) (aqp-1) (urine water channel). [source:swissprot;acc:p29972]	Squared	71346.6	95965.1	1.34505	
194	cyclin g2. [source:swissprot;acc:q16589]	Measured	17738.6	13995.7	1.26743	
194	eap30 subunit of ell complex. [source:refseq;acc:nm_007241]	Rooted	59.6399	51.9242	1.1486	
194	nuclear protein ukp68. [source:refseq;acc:nm_024824]	Ranked	227.491	199.458	1.14055	
195	cyclin g1 (cyclin g). [source:swissprot;acc:p51959]	Measured	17738.5	13995.6	1.26743	
195	huntingtin interacting protein c. [source:refseq;acc:nm_012272]	Ranked	227.971	200.417	1.13748	
195	liv-1 protein, estrogen regulated. [source:refseq;acc:nm_012319]	Rooted	59.6399	51.9242	1.1486	
195	translocon-associated protein, delta subunit precursor (trap-delta) (signal sequence receptor delta subunit) (ssr-delta). [source:swissprot;acc:p51571]	Squared	71062.4	95488.9	1.34373	
196	calsyntenin-1 precursor. [source:swissprot;acc:o94985]	Squared	71062.4	95488.9	1.34373	
196	forkhead box p4; fork head-related protein like a; winged-helix repressor foxp4. [source:refseq;acc:nm_138457]	Ranked	263.215	231.407	1.13745	
196	glutamate decarboxylase, 65 kda isoform (ec 4.1.1.15) (gad-65) (65 kda glutamic acid decarboxylase). [source:swissprot;acc:q05329]	Rooted	59.6399	51.9242	1.1486	
196	serine/threonine protein phosphatase 2a, 56 kda regulatory subunit, epsilon isoform (pp2a, b subunit, b' epsilon isoform) (pp2a, b subunit, b56 epsilon isoform) (pp2a, b subunit, pr61 epsilon isoform) (pp2a, b subunit, r5 epsilon isoform). [source:swissprot;acc:q16537]	Measured	17738.5	13995.7	1.26742	
197	forkhead box protein p1 (hspc215). [source:swissprot;acc:q9h334]	Ranked	263.215	231.407	1.13745	
197	gamma-soluble nsf attachment protein (snap-gamma) (n-ethylmaleimide- sensitive factor attachment protein, gamma). [source:swissprot;acc:q99747]	Rooted	59.6399	51.9242	1.1486	
197	serine/threonine protein phosphatase 2a, 56 kda regulatory subunit, alpha isoform (pp2a, b subunit, b' alpha isoform) (pp2a, b subunit, b56 alpha isoform) (pp2a, b subunit, pr61 alpha isoform) (pp2a, b subunit, r5 alpha isoform). [source:swissprot;acc:q15172]	Measured	17738.5	13995.7	1.26742	
197	succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial precursor (cybs) (succinate-ubiquinone reductase membrane anchor subunit) (qps2) (cii-4) (succinate dehydrogenase complex subunit d) (succinate-ubiquinone oxidoreductase cytochrome b small subunit). [source:swissprot;acc:o14521]	Squared	71062.4	95488.9	1.34373	
198	b-raf proto-oncogene serine/threonine-protein kinase (ec 2.7.1.-) (p94) (v-raf murine sarcoma viral oncogene homolog b1). [source:swissprot;acc:p15056]	Measured	17710.9	13977.7	1.26708	
198	forkhead box protein p3 (zinc finger protein jm2) (scurfin). [source:swissprot;acc:q9bzs1]	Ranked	263.191	231.394	1.13741	
198	glutamate decarboxylase, 67 kda isoform (ec 4.1.1.15) (gad-67) (67 kda glutamic acid decarboxylase). [source:swissprot;acc:q99259]	Rooted	59.6399	51.9242	1.1486	
198	protein-tyrosine phosphatase beta precursor (ec 3.1.3.48) (r-ptp- beta). [source:swissprot;acc:p23467]	Squared	71062.2	95488.6	1.34373	
199	palmitoyl-protein thioesterase 2 precursor (ec 3.1.2.22) (palmitoyl- protein hydrolase 2) (ppt-2) (g14). [source:swissprot;acc:q9umr5]	Ranked	242.735	276.059	1.13729	
199	serine/threonine protein phosphatase 2a, catalytic subunit, alpha isoform (ec 3.1.3.16) (pp2a-alpha) (replication protein c) (rp-c). [source:swissprot;acc:p05323]	Measured	4916.8	3888.18	1.26455	
199	source of immunodominant mhc-associated peptides; homolog of yeast stt3 gene. [source:refseq;acc:nm_178862]	Rooted	61.2893	70.3856	1.14842	
199	thymus-specific serine protease precursor (ec 3.4.-.-). [source:swissprot;acc:q9nqe7]	Squared	71062.2	95488.6	1.34373	
200	crk-associated substrate (p130cas) (breast cancer anti-estrogen resistance 1 protein). [source:swissprot;acc:p56945]	Measured	4808.79	6074.21	1.26315	
200	egf-like-domain, multiple 7; neu1 protein. [source:refseq;acc:nm_016215]	Ranked	242.718	276.018	1.1372	
200	gk001 protein. [source:refseq;acc:nm_020198]	Squared	71062.2	95488.6	1.34373	
200	oligosaccharyl transferase stt3 subunit homolog (b5) (integral membrane protein 1) (tmc). [source:swissprot;acc:p46977]	Rooted	61.2893	70.3856	1.14842	
201	calsyntenin-2 precursor. [source:swissprot;acc:q9h4d0]	Squared	71033.4	95440.2	1.3436	
201	dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kda subunit precursor (ec 2.4.1.119) (oligosaccharyl transferase 48 kda subunit) (ddost 48 kda subunit). [source:swissprot;acc:p39656]	Rooted	61.2893	70.3856	1.14842	
201	splicing factor 4 isoform b; rna-binding protein. [source:refseq;acc:nm_021164]	Ranked	240.133	211.382	1.13601	
201	t-complex protein 1, delta subunit (tcp-1-delta) (cct-delta) (stimulator of tar rna binding). [source:swissprot;acc:p50991]	Measured	4810.22	6075.04	1.26294	
202	calsyntenin-3 precursor. [source:swissprot;acc:q9bqt9]	Squared	71030.1	95434.8	1.34358	
202	enhancer of filamentation 1 (hef1) (crk-associated substrate-related protein) (cas-l) (casl) (pp105) (neural precursor cell expressed developmentally down-regulated 9). [source:swissprot;acc:q14511]	Measured	4812.01	6076.09	1.26269	
202	short transient receptor potential channel 4 (trpc4) (trp-related protein 4) (htrp-4) (htrp4). [source:swissprot;acc:q9ubn4]	Ranked	201.478	228.784	1.13553	
202	splicing factor 3b subunit 3 (spliceosome associated protein 130) (sap 130) (sf3b130) (pre-mrna splicing factor sf3b 130 kda subunit). [source:swissprot;acc:q15393]	Rooted	57.3527	65.7973	1.14724	
203	autophagy protein 12-like (apg12-like). [source:swissprot;acc:o94817]	Measured	11757.8	9352.11	1.25723	
203	ebp50-pdz interactor of 64 kda (epi64 protein). [source:swissprot;acc:q9bxi6]	Ranked	201.515	228.752	1.13516	
203	hpaii tiny  fragments locus 9c. [source:refseq;acc:nm_022727]	Squared	20988.2	15632.1	1.34263	
203	transcription factor dp-1 (e2f dimerization partner 1) (drtf1- polypeptide-1) (drtf1). [source:swissprot;acc:q14186]	Rooted	46.3291	53.1063	1.14628	
204	short transient receptor potential channel 5 (trpc5) (htrp-5) (htrp5). [source:swissprot;acc:q9ul62]	Ranked	201.515	228.752	1.13516	
204	small nuclear ribonucleoprotein e (snrnp-e) (sm protein e) (sm-e) (sme). [source:swissprot;acc:p08578]	Measured	4512.19	5655.52	1.25339	
204	small nuclear ribonucleoprotein e (snrnp-e) (sm protein e) (sm-e) (sme). [source:swissprot;acc:p08578]	Rooted	55.8956	63.9636	1.14434	
204	source of immunodominant mhc-associated peptides; homolog of yeast stt3 gene. [source:refseq;acc:nm_178862]	Squared	12326.4	16522.9	1.34045	
205	luc7-like 2; cgi-74 protein; cgi-59 protein. [source:refseq;acc:nm_016019]	Ranked	228.1	200.968	1.13501	
205	oligosaccharyl transferase stt3 subunit homolog (b5) (integral membrane protein 1) (tmc). [source:swissprot;acc:p46977]	Squared	12326.4	16522.9	1.34045	
205	small nuclear ribonucleoprotein f (snrnp-f) (sm protein f) (sm-f) (smf). [source:swissprot;acc:q15356]	Measured	4512.19	5655.52	1.25339	
205	small nuclear ribonucleoprotein f (snrnp-f) (sm protein f) (sm-f) (smf). [source:swissprot;acc:q15356]	Rooted	55.8956	63.9636	1.14434	
206	atlastin; guanylate-binding protein 3. [source:refseq;acc:nm_015915]	Measured	5720.25	4577.5	1.24965	
206	dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kda subunit precursor (ec 2.4.1.119) (oligosaccharyl transferase 48 kda subunit) (ddost 48 kda subunit). [source:swissprot;acc:p39656]	Squared	12326.4	16522.9	1.34045	

Legend:
- Rank is the rank after comparing the two networks
- Value Type describes how the microarray measurement was processed. Ranked indicates that we sorted all measurements and use the rank as red and green value. Measured is the normal measurement. Squared and Rooted are the square and square root respecitcely. This might be valueable to compensate for non linear light distributions.
- Network Comparison Type describes whether we divided the red and green types or whether we sutractcted them
- Interaction Map specifies whether we used only the hig hconfidence protein-protein interactions or also included the low confidence interactions.
- Filtered specifies whether we only included the high confidence microarry measurements or not.

- http://analysis.yellowcouch.org/