MK5 Microarray Data

These are the micro-array results of a differential gene expression microarray experiments and the subsequent analysis steps performed on them. The up/down- regulation ratio was obtained by measuring WT cells against MK5 activated cells. See material and methods for technical information as well as the data usage policy.

Ontology Breakdown

Below is a table that can help you break down the effect of the gene alteration on the bioloigcal system. To create the table below we went over all the ontology terms and counted the number of times a gene was listed in that specific class, or one of its children. As can be expected, each class will differ in its behavior. Some will have a strong average regulation, but will contain very few genes, while others will be very large and broad classes that will have a lower average regulation, but which will whos an overall effect with many genes in many subgroups being affected.

The best strategy to analyze such classes is to a) filter out all the classes for which we have too little data (less than 5 affected genes on the micro array measurement). This can be done by writing '>5' in the 'Affected Genes' field. SEcondly we might also want to look at one specific ontology at first (click on 'biological process' in the table.

Once this is done we can sort the data properly by placing the 'Average Regulation' to the left (click on the green arrows) and the '% Affected Genes' as a second. We also want to sort them descending (the grey arrow should be pointing downward).

Now we can go investigate the data by first look at broadly affected classes ('Would Be Affected Genes'>60) and progressively lowering this value (to something like 'Would Be Affected Genes'>20)

Navigation/Query Panel:
Click on the attribute name to hide/unhide it. The green arrows can be used to shift columns left/right. Exact word match is written as =..., regular expressions can be matched with ~.... To select all values larger or equal than use >.... This would be <... for values smaller or equal than. To select all values within a specific range use [...,...].

Average Regulation (ratio)	% Affected Genes	Class Size (#genes)	Would Be Affected (#genes)	Class	Class	affected	Microarray Measured (#genes)	Accession

Results: HTML CSV LaTeX

Showing element 125 to 174 of 328 in total

Average Regulation (ratio)	% Affected Genes	Class Size (#genes)	Would Be Affected (#genes)	Class	Class
2.3127479553222656	1.4970	398	6	behavior	biological process
2.312502180275164	2.3114	4136	96	cell communication
2.3059755516052247	2.2085	2892	64	intracellular organelle part	cellular component
	2.2017	2900		organelle part
2.3038527108075324	2.4081	2739	66	developmental process	biological process
2.3028621958649675	2.4798	2641	65	DNA binding	molecular function
2.299432896077633	1.8561	1060	20	integral to plasma membrane	cellular component
	1.8286	1077		intrinsic to plasma membrane
2.2985232734680174	2.5907	2698	70	regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolic process	biological process
2.28793443441391	2.6247	429	11	positive regulation of cellular metabolic process
	2.4631	456		positive regulation of metabolic process
2.2792132452352725	2.5000	3858	96	nucleic acid binding	molecular function
2.2737072706222534	2.2364	752	17	lipid metabolic process	biological process
2.2681225514251913	2.4354	7366	179	protein binding	molecular function
2.267966568470001	3.9735	164	7	transmembrane receptor protein tyrosine kinase signaling pathway	biological process
2.2671763740532787	2.3725	15762	374	cellular_component	cellular component
2.2670372059712043	2.4610	2913	72	regulation of cellular metabolic process	biological process
2.2664323395275208	2.4356	18539	452	all	universal
2.266423319776853	2.9091	972	28	cell fraction	cellular component
2.2609615601026096	2.4030	734	18	peptidase activity	molecular function
2.2604436332529243	2.2267	634	14	response to external stimulus	biological process
2.2601398825645447	1.5228	464	7	monovalent inorganic cation transport
2.259431820927244	2.3913	16757	401	molecular_function	molecular function
2.2576280551010304	2.3831	14761	352	cell part	cellular component
2.2540424267450967	1.9608	358	7	negative regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolic process	biological process
2.252760338363143	2.4522	11986	294	cellular process
2.248631090488074	2.4047	3018	73	regulation of metabolic process
2.246480769722174	2.4413	15431	377	biological_process
2.243723976612091	2.0202	762	15	macromolecule biosynthetic process
2.241470267334763	2.4400	13082	319	binding	molecular function
2.2348088707242693	2.5271	321	8	nervous system development	biological process
2.2326475211552212	1.9608	414		negative regulation of cellular metabolic process
2.231512101073014	3.0400	739	22	membrane fraction	cellular component
2.2257004976272583	1.8248	497	9	olfactory receptor activity	molecular function
2.2204760638150303	1.6923	826	14	extracellular region part	cellular component
2.220401409599516	2.2099	2403	53	zinc ion binding	molecular function
2.216642654982189	2.4279	7902	192	intracellular organelle	cellular component
	2.4275	7904		organelle
2.2164768129587173	3.4335	264	9	organ morphogenesis	biological process
2.215940025315356	2.4157	7030	170	intracellular membrane-bound organelle	cellular component
				membrane-bound organelle
2.210925724557651	2.3834	10046	239	intracellular part
2.2093183655257618	2.3335	5858	137	catalytic activity	molecular function
2.2058627247810363	2.0161	1164	23	negative regulation of cellular process	biological process
	1.8957	1238		negative regulation of biological process
2.201751559972763	1.8244	1284		G-protein coupled receptor activity	molecular function
2.201178240776062	3.3727	723	24	hydrolase activity, acting on ester bonds
2.195966625213623	1.9569	696	14	cytoskeletal part	cellular component
2.1927802711725235	3.4188	579	20	sequence-specific DNA binding	molecular function
2.1918027877807615	2.0661	569	12	metal ion transport	biological process

Legend:
- The Average Regulation (ratio) is calculated only for the measured genes in this gene ontology term
- % Affected Genes is the % of genes in this gene ontology class that have been affected by the Mk5 alteration.
- Would Be Affected (#genes) represents how many genes of the overall class would have been affected if we measured each of them.
- The Class Size (#genes) counts the number of genes listed under the specific gene ontology term.
- Class is the GO class description.
- The Class refers to the ontology category, which can be molecular_function, biological_process or cellular_component.
- The Microarray Measured (#genes) lists how many genes of the specific ontology term were measured.
- Accession is the GO accession key.