Scientific

MK5 Microarray Data


Affected Genes - Ontology Breakdown - Gene Enrichment - Proteome Analysis

These are the micro-array results of a differential gene expression microarray experiments and the subsequent analysis steps performed on them. The up/down- regulation ratio was obtained by measuring WT cells against MK5 activated cells. See material and methods for technical information as well as the data usage policy.

Ontology Breakdown

Below is a table that can help you break down the effect of the gene alteration on the bioloigcal system. To create the table below we went over all the ontology terms and counted the number of times a gene was listed in that specific class, or one of its children. As can be expected, each class will differ in its behavior. Some will have a strong average regulation, but will contain very few genes, while others will be very large and broad classes that will have a lower average regulation, but which will whos an overall effect with many genes in many subgroups being affected.

The best strategy to analyze such classes is to a) filter out all the classes for which we have too little data (less than 5 affected genes on the micro array measurement). This can be done by writing '>5' in the 'Affected Genes' field. SEcondly we might also want to look at one specific ontology at first (click on 'biological process' in the table.

Once this is done we can sort the data properly by placing the 'Average Regulation' to the left (click on the green arrows) and the '% Affected Genes' as a second. We also want to sort them descending (the grey arrow should be pointing downward).

Now we can go investigate the data by first look at broadly affected classes ('Would Be Affected Genes'>60) and progressively lowering this value (to something like 'Would Be Affected Genes'>20)


Navigation/Query Panel:
Click on the attribute name to hide/unhide it. The green arrows can be used to shift columns left/right. Exact word match is written as =..., regular expressions can be matched with ~.... To select all values larger or equal than use >.... This would be <... for values smaller or equal than. To select all values within a specific range use [...,...].
% Affected Genes Average Regulation (ratio) Would Be Affected (#genes) Class Class Size (#genes) Class affected Microarray Measured (#genes) Accession
Results: HTML CSV LaTeX Showing element 1 to 50 of 173 in total
Class: biological process
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2.6894871592521667\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 7\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent muscle development\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 117\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 5.7143\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 2.1655175536870956\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 9\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent dephosphorylation\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 158\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 5.1546\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 2.329086923599243\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 6\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent embryonic morphogenesis\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 107\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 5.0000\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 2.6329536702897816\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 11\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent response to cold\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 226\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 4.7244\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 1.632481078306834\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 7\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent mitosis\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 142\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 4.7059\vspace{3pt}}&\multirow{2}{*}{\parbox{\wcB}{\vspace{3pt}\noindent 2.4113256633281708\vspace{3pt}}}&\multirow{2}{*}{\parbox{\wcC}{\vspace{3pt}\noindent 10\vspace{3pt}}}&\parbox{\wcD}{\vspace{3pt}\noindent response to freezing\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 216\vspace{3pt}}\\\cline{1-1}\cline{4-5} \parbox{\wcA}{\vspace{3pt}\noindent 4.6784\vspace{3pt}}&&&\parbox{\wcD}{\vspace{3pt}\noindent homoiothermy\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 217\vspace{3pt}}\\\hline 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2.3925783783197403\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 9\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent enzyme linked receptor protein signaling pathway\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 230\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 3.7234\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 1.8230999537876673\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 8\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent RNA splicing\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 208\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 3.5604\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 1.8924930147502734\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 27\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent cell cycle process\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 746\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 3.4335\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 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12\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent alcohol metabolic process\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 378\vspace{3pt}}\\\hline \multirow{2}{*}{\parbox{\wcA}{\vspace{3pt}\noindent 3.0968\vspace{3pt}}}&\multirow{2}{*}{\parbox{\wcB}{\vspace{3pt}\noindent 2.4829982866843543\vspace{3pt}}}&\multirow{2}{*}{\parbox{\wcC}{\vspace{3pt}\noindent 28\vspace{3pt}}}&\parbox{\wcD}{\vspace{3pt}\noindent phosphate metabolic process\vspace{3pt}}&\multirow{2}{*}{\parbox{\wcE}{\vspace{3pt}\noindent 890\vspace{3pt}}}\\\cline{4-4} &&&\parbox{\wcD}{\vspace{3pt}\noindent phosphorus metabolic process\vspace{3pt}}&\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 3.0405\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 2.470501118236118\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 52\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent protein modification process\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 1721\vspace{3pt}}\\\hline \multirow{2}{*}{\parbox{\wcA}{\vspace{3pt}\noindent 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wounding\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 358\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 2.9070\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 2.844642996788025\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 6\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent nucleocytoplasmic transport\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 206\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 2.8939\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 2.3975469536251492\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 10\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent positive regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolic process\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 347\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 2.8736\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 2.530326783657074\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 12\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent response to abiotic stimulus\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 416\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 2.8646\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 2.5862315134568648\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 13\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent ubiquitin cycle\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 443\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 2.8333\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 2.1418599311043236\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 19\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent anatomical structure morphogenesis\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 686\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 2.8249\vspace{3pt}}&\multirow{2}{*}{\parbox{\wcB}{\vspace{3pt}\noindent 2.7829527854919434\vspace{3pt}}}&\multirow{3}{*}{\parbox{\wcC}{\vspace{3pt}\noindent 6\vspace{3pt}}}&\parbox{\wcD}{\vspace{3pt}\noindent visual perception\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 211\vspace{3pt}}\\\cline{1-1}\cline{4-5} \parbox{\wcA}{\vspace{3pt}\noindent 2.8090\vspace{3pt}}&&&\parbox{\wcD}{\vspace{3pt}\noindent sensory perception of light stimulus\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 212\vspace{3pt}}\\\cline{1-2}\cline{4-5} \parbox{\wcA}{\vspace{3pt}\noindent 2.7174\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 3.0316067695617677\vspace{3pt}}&&\parbox{\wcD}{\vspace{3pt}\noindent microtubule-based process\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 231\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 2.6820\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 2.7678764888218472\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 33\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent intracellular signaling cascade\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 1217\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 2.6596\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 1.6556044340133667\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 6\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent anion transport\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 229\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 2.6362\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 2.630213709672292\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 42\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent transcription\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 1588\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 2.6247\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 2.28793443441391\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 11\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent positive regulation of cellular metabolic process\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 429\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 2.6245\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 2.3178554457061145\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 69\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent regulation of transcription\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 2628\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 2.6235\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 2.3607054107329426\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 20\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent protein transport\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 768\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 2.6201\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 2.6820639967918396\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 19\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent immune response\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 735\vspace{3pt}}\\\hline \parbox{\wcA}{\vspace{3pt}\noindent 2.6103\vspace{3pt}}&\parbox{\wcB}{\vspace{3pt}\noindent 2.1811264465595115\vspace{3pt}}&\parbox{\wcC}{\vspace{3pt}\noindent 33\vspace{3pt}}&\parbox{\wcD}{\vspace{3pt}\noindent anatomical structure development\vspace{3pt}}&\parbox{\wcE}{\vspace{3pt}\noindent 1273\vspace{3pt}}\\\hline nd{longtable}
Legend:
- The Average Regulation (ratio) is calculated only for the measured genes in this gene ontology term
- % Affected Genes is the % of genes in this gene ontology class that have been affected by the Mk5 alteration.
- Would Be Affected (#genes) represents how many genes of the overall class would have been affected if we measured each of them.
- The Class Size (#genes) counts the number of genes listed under the specific gene ontology term.
- Class is the GO class description.
- The Class refers to the ontology category, which can be molecular_function, biological_process or cellular_component.
- The Microarray Measured (#genes) lists how many genes of the specific ontology term were measured.
- Accession is the GO accession key.

- http://analysis.yellowcouch.org/