MK5 Microarray Data

These are the micro-array results of a differential gene expression microarray experiments and the subsequent analysis steps performed on them. The up/down- regulation ratio was obtained by measuring WT cells against MK5 activated cells. See material and methods for technical information as well as the data usage policy.

Ontology Breakdown

Below is a table that can help you break down the effect of the gene alteration on the bioloigcal system. To create the table below we went over all the ontology terms and counted the number of times a gene was listed in that specific class, or one of its children. As can be expected, each class will differ in its behavior. Some will have a strong average regulation, but will contain very few genes, while others will be very large and broad classes that will have a lower average regulation, but which will whos an overall effect with many genes in many subgroups being affected.

The best strategy to analyze such classes is to a) filter out all the classes for which we have too little data (less than 5 affected genes on the micro array measurement). This can be done by writing '>5' in the 'Affected Genes' field. SEcondly we might also want to look at one specific ontology at first (click on 'biological process' in the table.

Once this is done we can sort the data properly by placing the 'Average Regulation' to the left (click on the green arrows) and the '% Affected Genes' as a second. We also want to sort them descending (the grey arrow should be pointing downward).

Now we can go investigate the data by first look at broadly affected classes ('Would Be Affected Genes'>60) and progressively lowering this value (to something like 'Would Be Affected Genes'>20)

Navigation/Query Panel:
Click on the attribute name to hide/unhide it. The green arrows can be used to shift columns left/right. Exact word match is written as =..., regular expressions can be matched with ~.... To select all values larger or equal than use >.... This would be <... for values smaller or equal than. To select all values within a specific range use [...,...].

Would Be Affected (#genes)	Average Regulation (ratio)	% Affected Genes	Class Size (#genes)	Class	Class	affected	Microarray Measured (#genes)	Accession

Results: HTML CSV LaTeX

Showing element 101 to 150 of 173 in total

Class  : biological process

Would Be Affected (#genes)	Average Regulation (ratio)	% Affected Genes	Class Size (#genes)	Class
15	3.0782742261886598	2.2026	680	defense response
	2.46775693159837	2.9680	499	regulation of transcription from RNA polymerase II promoter
	2.243723976612091	2.0202	762	macromolecule biosynthetic process
16	2.539123611790793	2.5045	644	organ development
	2.4382004340489707	2.2514	732	sensory perception
		2.2181	741	neurological process
	2.0980815972600664	1.9204	832	RNA metabolic process
	2.0103142169805674	2.5048	623	homeostatic process
		2.3679	660	regulation of biological quality
17	2.5815784772237143	2.5554	675	protein amino acid phosphorylation
	2.5267262917298536	1.9118	884	DNA metabolic process
	2.2737072706222534	2.2364	752	lipid metabolic process
	2.16537378515516	2.0202	840	intracellular transport
	1.9759902954101562	2.1417	800	proteolysis
	1.8383450422968184	2.1407	806	biological adhesion
				cell adhesion
	1.714063827196757	2.0325	851	multicellular organismal development
18	2.6417386531829834	2.5890	711	phosphorylation
19	2.6820639967918396	2.6201	735	immune response
	2.1418599311043236	2.8333	686	anatomical structure morphogenesis
20	2.6115847550905666	2.2184	879	immune system process
	2.3607054107329426	2.6235	768	protein transport
		2.4781	810	establishment of protein localization
	2.1725611826952766	2.2398	914	establishment of cellular localization
21	2.5005394551489086	1.8711	1118	cellular developmental process
22	2.02202084991667	2.2167	974	ion transport
23	2.2058627247810363	2.0161	1164	negative regulation of cellular process
		1.8957	1238	negative regulation of biological process
	1.8484420895576477	4.3573	535	regulation of progression through cell cycle
		4.3197	539	regulation of cell cycle
25	1.9621534997766668	2.4123	1036	positive regulation of cellular process
26	2.644248050451279	2.1413	1204	organelle organization and biogenesis
	1.9786490954850848	1.8357	1417	biosynthetic process
27	1.8924930147502734	3.5604	746	cell cycle process
28	2.4829982866843543	3.0968	890	phosphate metabolic process
				phosphorus metabolic process
29	2.017735466957092	2.4655	1165	positive regulation of biological process
33	2.7678764888218472	2.6820	1217	intracellular signaling cascade
	2.1811264465595115	2.6103	1273	anatomical structure development
34	2.416864479581515	2.3483	1453	G-protein coupled receptor protein signaling pathway
37	2.403050871690114	3.0060	1226	response to stress
42	2.630213709672292	2.6362	1588	transcription
	2.43051519464044	2.0821	2008	cellular component organization and biogenesis
43	2.4479837675352356	2.9529	1442	post-translational protein modification
46	2.395317418234689	2.2307	2077	cell surface receptor linked signal transduction
48	1.9769652134332902	2.0945	2294	multicellular organismal process
52	2.470501118236118	3.0405	1721	protein modification process
		2.9393	1780	biopolymer modification
62	2.3732517415826972	2.5375	2448	regulation of transcription, DNA-dependent
66	2.3038527108075324	2.4081	2739	developmental process

Legend:
- The Average Regulation (ratio) is calculated only for the measured genes in this gene ontology term
- % Affected Genes is the % of genes in this gene ontology class that have been affected by the Mk5 alteration.
- Would Be Affected (#genes) represents how many genes of the overall class would have been affected if we measured each of them.
- The Class Size (#genes) counts the number of genes listed under the specific gene ontology term.
- Class is the GO class description.
- The Class refers to the ontology category, which can be molecular_function, biological_process or cellular_component.
- The Microarray Measured (#genes) lists how many genes of the specific ontology term were measured.
- Accession is the GO accession key.