MK5 Microarray Data

These are the micro-array results of a differential gene expression microarray experiments and the subsequent analysis steps performed on them. The up/down- regulation ratio was obtained by measuring WT cells against MK5 activated cells. See material and methods for technical information as well as the data usage policy.

Ontology Breakdown

Below is a table that can help you break down the effect of the gene alteration on the bioloigcal system. To create the table below we went over all the ontology terms and counted the number of times a gene was listed in that specific class, or one of its children. As can be expected, each class will differ in its behavior. Some will have a strong average regulation, but will contain very few genes, while others will be very large and broad classes that will have a lower average regulation, but which will whos an overall effect with many genes in many subgroups being affected.

The best strategy to analyze such classes is to a) filter out all the classes for which we have too little data (less than 5 affected genes on the micro array measurement). This can be done by writing '>5' in the 'Affected Genes' field. SEcondly we might also want to look at one specific ontology at first (click on 'biological process' in the table.

Once this is done we can sort the data properly by placing the 'Average Regulation' to the left (click on the green arrows) and the '% Affected Genes' as a second. We also want to sort them descending (the grey arrow should be pointing downward).

Now we can go investigate the data by first look at broadly affected classes ('Would Be Affected Genes'>60) and progressively lowering this value (to something like 'Would Be Affected Genes'>20)

Navigation/Query Panel:
Click on the attribute name to hide/unhide it. The green arrows can be used to shift columns left/right. Exact word match is written as =..., regular expressions can be matched with ~.... To select all values larger or equal than use >.... This would be <... for values smaller or equal than. To select all values within a specific range use [...,...].

Average Regulation (ratio)	% Affected Genes	Would Be Affected (#genes)	affected	Class	Class Size (#genes)	Class	Microarray Measured (#genes)	Accession

Results: HTML CSV LaTeX

Showing element 25 to 74 of 5007 in total

Class  : biological process

Average Regulation (ratio)	% Affected Genes	Would Be Affected (#genes)	Class	Class Size (#genes)
4.7113165855407715	1.0638	1	carbohydrate catabolic process	121
	0.9615		glucose metabolic process	130
	0.6944		hexose metabolic process	174
	0.6623		monosaccharide metabolic process	182
4.620415210723877	2.7027		protein amino acid N-linked glycosylation	40
4.601345539093018	1.0811	2	reproductive process	225
4.506189823150635	5.8824	1	phagocytosis	18
4.492823123931885	6.6667		negative regulation of gene expression, epigenetic	20
	2.2222		regulation of gene expression, epigenetic	53
4.407965660095215	1.3333		neuropeptide signaling pathway	100
4.105485280354817	5.4545	4	humoral immune response	80
4.079947471618652	3.4483	1	insulin receptor signaling pathway	32
4.046511650085449	50.0000	2	nuclear migration	3
	33.3333	1	nuclear membrane organization and biogenesis
	25.0000	2	establishment of nucleus localization	6
	12.5000	1	mitotic spindle organization and biogenesis	9
4.037245273590088	14.2857		transmembrane receptor protein tyrosine phosphatase signaling pathway	7
3.9217323064804077	40.0000	2	embryonic skeletal morphogenesis	5
	18.1818		skeletal morphogenesis	13
3.895843982696533	1.0309	1	Wnt receptor signaling pathway	109
3.819567561149597	3.2258	4	regulation of cell growth	133
3.8173599243164062	2.9412	1	transcription initiation from RNA polymerase II promoter	39
	1.7857		transcription initiation	68
3.721451759338379	4.4444	2	chromatin remodeling	53
3.7029240131378174	50.0000	1	myoblast migration	2
	11.1111		thymus development	10
	2.7778		striated muscle development	38
	1.9608		hemopoietic or lymphoid organ development	66
3.595149040222168	7.6923	3	negative regulation of cell growth	41
	5.8824		negative regulation of growth	57
3.594250440597534	0.9259	2	cell migration	248
3.575074315071106	4.4444		axonogenesis	49
	3.7736		cell part morphogenesis	59
			cell projection morphogenesis
			neurite morphogenesis
3.485576868057251	1.4286	1	axon guidance	80
3.4853661954402924	5.2632	5	microtubule cytoskeleton organization and biogenesis	87
3.400171995162964	1.8987	3	cellular structure morphogenesis	176
3.3986358642578125	2.4691	5	second-messenger-mediated signaling	201
3.398496985435486	10.0000	2	spindle organization and biogenesis	23
3.3942748308181763	4.3478		phosphoinositide metabolic process	52
	3.1746		glycerophospholipid metabolic process	72
	1.5038		phospholipid metabolic process	155
3.367248058319092	100.0000	1	negative regulation of antiviral response	1
			negative regulation of antiviral response by host
			positive regulation of viral protein levels in host cell
			regulation of viral protein levels in host cell
	50.0000	2	positive regulation of viral genome replication	3
			regulation of antiviral response by host	4
			regulation of viral transcription	3

Legend:
- The Average Regulation (ratio) is calculated only for the measured genes in this gene ontology term
- % Affected Genes is the % of genes in this gene ontology class that have been affected by the Mk5 alteration.
- Would Be Affected (#genes) represents how many genes of the overall class would have been affected if we measured each of them.
- The Class Size (#genes) counts the number of genes listed under the specific gene ontology term.
- Class is the GO class description.
- The Class refers to the ontology category, which can be molecular_function, biological_process or cellular_component.
- The Microarray Measured (#genes) lists how many genes of the specific ontology term were measured.
- Accession is the GO accession key.