2D Gel Image Registration

A task often necessary when working with 2 dimensional electrophoretic gels is finding similarities between the two gels. By overlaying different gels in an appropriate way it is possible to naturally investigate the similarity between two gels.

The image presents the 'best possible overlay' of two gel images. The red spots are from one gel, the blue spots are from another gel. Places where the color of the spot is black instead of blue or red show locations where the two spots match. In the picture some of these spots are marked with a red circle.

Based on this kind of overlays it is further possible to group large sets of gels automatically into clusters based on their overlay similarities.